Gajanayaka Navindu Dinara, Jo Eunyoung, Bandara Minthari Sakethanika, Marasinghe Svini Dileepa, Bawkar Chinmayee, Lee Yeon-Ju, Park Gun-Hoo, Oh Chulhong, Lee Youngdeuk
Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju-si 63349, Republic of Korea.
Department of Marine Technology & Convergence Engineering, KIOST School, University of Science and Technology, Daejeon 34113, Republic of Korea.
Mar Drugs. 2024 Dec 23;22(12):577. doi: 10.3390/md22120577.
Green algae, particularly species, are rich in complex polysaccharides, such as ulvan, which have significant potential for biotechnological applications. However, the biochemical properties of ulvan depolymerised products remain underexplored. The enzymatic depolymerisation of ulvan has garnered attention owing to its cost advantages over alternative methods. Nevertheless, the biochemical characterisation of ulvan lyases, specifically those belonging to the polysaccharide lyase family 25 (PL25), is limited. In this study, we identified and biochemically characterised a novel PL25 ulvan lyase, PaUL25, which functions optimally at pH 10. Additionally, we explored the alpha (α)-glucosidase inhibitory properties of ulvan depolymerised products. PaUL25 exhibited optimum activity at 35 °C in Tris-HCl buffer (pH 10). Moreover, enzyme activity was enhanced by more than 150% in the presence of Mn metal ions at and below concentrations of 10 mM. The endolytic action of PaUL25 produced ulvan oligosaccharides with degrees of polymerisation of 2 and 4 as its end products. Partially and completely hydrolysed ulvan oligosaccharides exhibited α-glucosidase inhibitory activity, with half inhibitory concentration IC values of 3.21 ± 0.13 and 2.51 ± 0.19 mg/mL, respectively. These findings expand our understanding of PL25 and highlight the pharmaceutical potential of ulvan oligosaccharides, particularly as antidiabetic agents.
绿藻,尤其是某些种类,富含复杂多糖,如岩藻聚糖,其在生物技术应用方面具有巨大潜力。然而,岩藻聚糖解聚产物的生化特性仍未得到充分探索。由于与其他方法相比具有成本优势,岩藻聚糖的酶促解聚受到了关注。尽管如此,岩藻聚糖裂解酶的生化特性,特别是那些属于多糖裂解酶家族25(PL25)的裂解酶,仍然有限。在本研究中,我们鉴定并对一种新型PL25岩藻聚糖裂解酶PaUL25进行了生化特性分析,该酶在pH 10时功能最佳。此外,我们还探索了岩藻聚糖解聚产物的α-葡萄糖苷酶抑制特性。PaUL25在Tris-HCl缓冲液(pH 10)中于35°C时表现出最佳活性。此外,在10 mM及以下浓度的锰金属离子存在下,酶活性提高了150%以上。PaUL25的内切作用产生了聚合度为2和4的岩藻寡糖作为终产物。部分水解和完全水解的岩藻寡糖均表现出α-葡萄糖苷酶抑制活性,半抑制浓度IC值分别为3.21±0.13和2.51±0.19 mg/mL。这些发现扩展了我们对PL25的理解,并突出了岩藻寡糖的药用潜力,特别是作为抗糖尿病药物的潜力。