Wang Huiling, Wang Xiaohan, Han Huiling, Yu Quanlin, Tan Xinmiao, Liu Junlong, Zhao Yiting, Sun Weiming
College of Agriculture and Biotechnology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China.
Hebei Key Laboratory of Crop Stress Biology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China.
Toxins (Basel). 2024 Dec 18;16(12):548. doi: 10.3390/toxins16120548.
is the main pathogen of peanut pod rot in China. To investigate the type of toxin and its pathogenic mechanism, a macrolide, brefeldin A, was isolated. The structure of the compound was identified by 1D and 2D nuclear magnetic resonance (NMR) and high-resolution electrospray ionization-mass spectrometry (HR-ESI-MS). At the same time, the content of the compound in healthy and diseased peanut capsules was detected, and its plant toxicity to radish, mung bean, rice, and peanut seed radicle elongation and pathogenicity to peanut pod rot were evaluated. The results showed that brefeldin A at 50 μg/mL could significantly inhibit the radicle elongation of rice seeds. Brefeldin A was detected only in pods with peanut rot. Injecting 2 mg/mL brefeldin A solution into peanut pods caused the severe decay of peanut pods at the R3R4 stage, which is consistent with the symptoms of peanut rot.
是中国花生荚腐病的主要病原菌。为了研究毒素类型及其致病机制，分离出一种大环内酯类化合物布雷菲德菌素A。通过一维和二维核磁共振（NMR）以及高分辨率电喷雾电离质谱（HR-ESI-MS）对该化合物的结构进行了鉴定。同时，检测了该化合物在健康和患病花生荚中的含量，并评估了其对萝卜、绿豆、水稻以及花生种子胚根伸长的植物毒性和对花生荚腐病的致病性。结果表明，50μg/mL的布雷菲德菌素A能显著抑制水稻种子胚根的伸长。仅在患花生腐病的荚中检测到布雷菲德菌素A。向花生荚中注射2mg/mL的布雷菲德菌素A溶液会导致处于R3-R4阶段的花生荚严重腐烂，这与花生腐病的症状一致。
