用于模拟冠状动脉痉挛的三种诱导多能干细胞系的产生与鉴定
Generation and characterization of three induced pluripotent stem cell lines for modeling coronary artery vasospasm.
作者信息
Ross Tacco Ines, Olshausen Joseph, Chan Tse Yuan, Turbes Naima, Hung Ming-Yow, Yeh Chi-Tai, Nguyen Patricia K, Sallam Karim, Sayed Nazish, Chen Ian Y
机构信息
Cardiology Section, Medical Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Radiology Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA; Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA.
Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA; Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA.
出版信息
Stem Cell Res. 2025 Feb;82:103644. doi: 10.1016/j.scr.2024.103644. Epub 2024 Dec 24.
Coronary artery vasospasm (CAV) is characterized by transient constriction of epicardial coronary arteries leading to angina. Its disease mechanisms are multifactorial but has centered mostly on endothelial dysfunction and smooth muscle hyperreactivity. To facilitate the investigation of these mechanisms in cell culture, we generated and characterized three induced pluripotent stem cell (iPSC) lines from patients with CAV. These lines demonstrated normal morphology and karyotypes, robust expression of pluripotency markers, and ability for tri-lineage differentiation. Further differentiation of these cell lines into endothelial and smooth muscle cells will allow mechanistic investigation of their relative contributions to CAV in cell culture.
冠状动脉痉挛（CAV）的特征是心外膜冠状动脉短暂收缩导致心绞痛。其发病机制是多因素的，但主要集中在内皮功能障碍和平滑肌高反应性上。为便于在细胞培养中研究这些机制，我们从CAV患者中生成并鉴定了三条诱导多能干细胞（iPSC）系。这些细胞系表现出正常的形态和核型、多能性标志物的强表达以及三系分化能力。将这些细胞系进一步分化为内皮细胞和平滑肌细胞，将有助于在细胞培养中对它们在CAV中的相对作用进行机制研究。
Zotero 插件