León Madrazo Anaí, Quintana Owen Patricia, Pérez Mendoza Gerardo, Segura Campos Maira Rubi
Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Periférico Norte Km. 33.5, Tablaje Catastral 13615, Col. Chuburná de Hidalgo Inn, Mérida, 97203, Yucatán, México.
Centro de Investigación y de Estudios Avanzados (CINVESTAV), Unidad Mérida Km 6, Cordemex, Mérida, 97310, Yucatán, México.
Plant Foods Hum Nutr. 2024 Dec 28;80(1):22. doi: 10.1007/s11130-024-01240-4.
The increasing concern over microbial resistance to conventional antimicrobial agents used in food preservation has led to growing interest in plant-derived antimicrobial peptides (AMPs) as alternative solutions. In this study, the antimicrobial mechanisms of chia seed-derived peptides YACLKVK, KLKKNL, KLLKKYL, and KKLLKI were investigated against Staphylococcus aureus (SA) and Escherichia coli (EC). Fluorometric assays and scanning electron microscopy (SEM) demonstrated that the peptides disrupt bacterial membranes, with propidium iodide (PI) uptake reaching 72.34% in SA, calcein release of 98.27%, and N-phenyl-1-naphthylamine (NPN) uptake of 84.35% in EC. Increased membrane permeabilization was observed at concentrations above 5 mg/mL. SEM results further confirmed significant morphological changes, supporting the observed membrane damage. Additionally, the peptides showed intracellular activity by altering EC DNA mobility, suggesting a secondary antimicrobial mechanism through DNA interaction. These results indicate that the peptides are promising antimicrobials with potential mechanisms beyond membrane disruption, highlighting the need for further research to comprehensively understand their antimicrobial mechanisms.
对食品保鲜中使用的传统抗菌剂产生的微生物耐药性的日益关注，引发了人们对植物源抗菌肽（AMPs）作为替代解决方案的兴趣日益浓厚。在本研究中，研究了奇亚籽衍生肽YACLKVK、KLKKNL、KLLKKYL和KKLLKI对金黄色葡萄球菌（SA）和大肠杆菌（EC）的抗菌机制。荧光测定法和扫描电子显微镜（SEM）表明，这些肽会破坏细菌膜，在SA中碘化丙啶（PI）摄取率达到72.34%，在EC中钙黄绿素释放率为98.27%，N-苯基-1-萘胺（NPN）摄取率为84.35%。在浓度高于5mg/mL时观察到膜通透性增加。SEM结果进一步证实了显著的形态变化，支持了观察到的膜损伤。此外，这些肽通过改变EC DNA迁移率显示出细胞内活性，表明通过DNA相互作用存在第二种抗菌机制。这些结果表明，这些肽是有前景的抗菌剂，其潜在机制超出了膜破坏，突出了进一步研究以全面了解其抗菌机制的必要性。
