Fan Yonghong, Zhang Feiran, He Kun, Yu Dan, Chen Haoming, Tian Da, Shi Yixiao, Li Zhen, Wang Xiaomei
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Research Institute of Petroleum Exploration and Development, PetroChina, Beijing 100083, China.
Bioresour Technol. 2025 Mar;419:132007. doi: 10.1016/j.biortech.2024.132007. Epub 2024 Dec 27.
The rapid growth of global energy demand accelerates the development of sustainable, clean, and renewable energy sources. Biohydrogen production, driven by functional microorganisms, offers a promising solution. Multiple species of bacteria, fungi, microalgae, and archaea were able to produce hydrogen. This study reviewed the typical strains, together with their hydrogen-production mechanisms, e.g., bio-photolysis, photo fermentation, and dark fermentation. Bacteria (e.g., purple non-sulfur bacteria) and microalgae (e.g., cyanobacteria) have been widely investigated, with respect to the limited fungi and archaea. It showed that temperature, pH, and substrate availability could all substantially influence the efficiency of biohydrogen production. Meanwhile, photo and dark fermentations are favored for future possible industrial applications. Furthermore, this review summarized practical applications of biohydrogen production, such as applications of bioreactors, waste treatments, and integrated systems for hydrogen production, highlighting the importance of functional microorganisms in advancing biohydrogen technology under global energy crisis.
全球能源需求的快速增长加速了可持续、清洁和可再生能源的发展。由功能微生物驱动的生物制氢提供了一个有前景的解决方案。多种细菌、真菌、微藻和古菌都能够产生氢气。本研究综述了典型菌株及其产氢机制，如生物光解、光发酵和暗发酵。细菌（如紫色非硫细菌）和微藻（如蓝藻）已得到广泛研究，而真菌和古菌的研究相对有限。结果表明，温度、pH值和底物可用性都会对生物制氢效率产生重大影响。同时，光发酵和暗发酵在未来可能的工业应用中更受青睐。此外，本综述总结了生物制氢的实际应用，如生物反应器的应用、废物处理以及制氢集成系统，强调了功能微生物在全球能源危机下推动生物制氢技术发展中的重要性。
