Li Jin, Wang Jiankun, Wu Jin, Wang Xiaoling
School of Mechanical Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.
Arch Microbiol. 2024 Dec 30;207(1):19. doi: 10.1007/s00203-024-04224-9.
During the self-healing process of Bacillus subtilis biofilms on a solid MSgg substrate, large-scale ordered clusters emerge within the biofilm, providing an invasive advantages. To investigate the self-healing mechanism, an agent-based model is employed to simulate the self-healing processes of biofilms at two ages. The study reveals that a uniform cell distribution facilitates the healing of biofilm incisions. The nutrient diffusion rate within the biofilm and the elastic modulus (comprising cell and EPS) play a dominant role in the healing of circumferential incisions, while the diffusion rate outside the biofilm governs the healing of radial and penetrating incisions. These influencing factors can adjust cellular ordering, providing valuable insights for controlling the self-healing of Bacillus subtilis biofilms.
在枯草芽孢杆菌生物膜在固体MSgg底物上的自我修复过程中，生物膜内会出现大规模有序簇，这提供了一种侵入优势。为了研究自我修复机制，采用基于主体的模型来模拟两个龄期生物膜的自我修复过程。研究表明，均匀的细胞分布有利于生物膜切口的愈合。生物膜内的营养物质扩散速率和弹性模量（包括细胞和胞外聚合物）在圆周切口的愈合中起主导作用，而生物膜外的扩散速率则控制径向和穿透性切口的愈合。这些影响因素可以调节细胞的有序性，为控制枯草芽孢杆菌生物膜的自我修复提供有价值的见解。
