Wang Shu, Li Yulin, Gao Jingfan, Lin Jia, Jin Xin, Zhang Hong
Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Harbin Medical University, Harbin, China.
Sci Rep. 2025 Jan 4;15(1):754. doi: 10.1038/s41598-024-84742-0.
To develop an atrophic Meibomian Gland Dysfunction (MGD) animal model via liquid nitrogen cryotherapy, the eyelid edges of C57 mice exposure to liquid nitrogen for 30 s. Morphology of MG and ocular surface were assessed using stereomicroscopy and a slit lamp microscope at multiple time points post-injury. Acinar loss and atrophy were observed from day 7, with increased inflammation and apoptosis, and decreased proliferation in acinar cells. Corneal epithelial defects appeared after day 14. Liquid nitrogen induced selective damage to meibomian acinar cells, simulating MGD pathology effectively, with peak effects at day 21, providing a relevant model for atrophic MGD research.
为了通过液氮冷冻疗法建立萎缩性睑板腺功能障碍（MGD）动物模型，将C57小鼠的眼睑边缘暴露于液氮中30秒。在损伤后的多个时间点，使用体视显微镜和裂隙灯显微镜评估睑板腺（MG）和眼表的形态。从第7天开始观察到腺泡丢失和萎缩，同时炎症和细胞凋亡增加，腺泡细胞增殖减少。第14天后出现角膜上皮缺损。液氮诱导睑板腺泡细胞选择性损伤，有效模拟MGD病理，在第21天达到峰值效应，为萎缩性MGD研究提供了相关模型。
