Wang Yanlan, Zhou Shiqi, Wang Xiaoshuang, Lu Dongheng, Yang Jinghong, Lu Yu, Fan Xiaolei, Li Changhao, Wang Yan
Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, China.
State Key Laboratory of Digital Medical Engineering, Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Sanya, 572025, China.
Biomaterials. 2025 Jun;317:123077. doi: 10.1016/j.biomaterials.2024.123077. Epub 2024 Dec 31.
Post-extraction alveolar bone resorption invariably compromises implant placement and aesthetic restoration outcomes. Current non-resorbable membranes exhibit limited efficacy in alveolar ridge preservation (ARP) due to insufficient cell recruitment and osteoinductive capabilities. Herein, we introduce a multifunctional electroactive membrane (PPy-BTO/P(VDF-TrFE), PB/PT) designed to spatiotemporally regulate cell migration and osteogenesis, harmonizing with the socket healing process. Initially, the membrane's endogenous-level surface potential recruits stem cells from the socket. Subsequently, adherent cell-migration-triggered forces generate on-demand piezopotential, stimulating intracellular calcium ion fluctuations and activating the Ca/calcineurin/NFAT1 signaling pathway via Cav3.2 channels. This enhances cell motility and osteogenic differentiation predominantly in the coronal socket region, counteracting the natural healing trajectory. The membrane's self-powered energy supply, proportional to cell migration velocity and manifested as nanoparticle deformation, mitigates ridge shrinkage, both independently and in conjunction with bone grafts. This energy-autonomous membrane, based on the spatiotemporal modulation of cell motility, presents a novel approach for in-situ ARP treatment and the development of 4D bionic scaffolds.
拔牙后牙槽骨吸收总是会影响种植体植入和美学修复效果。由于细胞募集不足和骨诱导能力有限，目前的不可吸收膜在牙槽嵴保存（ARP）方面疗效有限。在此，我们介绍一种多功能电活性膜（聚吡咯-钛酸钡/聚（偏氟乙烯-三氟乙烯），PB/PT），其设计用于时空调节细胞迁移和成骨，与牙槽窝愈合过程相协调。最初，膜的内源性表面电位从牙槽窝招募干细胞。随后，粘附细胞迁移触发的力产生按需压电势，刺激细胞内钙离子波动，并通过Cav3.2通道激活Ca/钙调神经磷酸酶/NFAT1信号通路。这主要增强了牙槽窝冠部区域的细胞运动性和成骨分化，抵消了自然愈合轨迹。膜的自供电能量供应与细胞迁移速度成正比，并表现为纳米颗粒变形，可独立或与骨移植联合减轻牙槽嵴萎缩。这种基于细胞运动性时空调节的能量自主膜，为原位ARP治疗和4D仿生支架的开发提供了一种新方法。
