genes are essential for plant development and secondary metabolism. The majority of genes within a genome exist in a gene family, each with specific functions. Ginseng is an herb used in medicine for its potential health benefits. The gene family in Jilin ginseng has not been studied. This study investigated the evolution and structural and functional diversification of the gene family using bioinformatics and analyzed gene expression through methyl jasmonate (MeJA) regulation. The results revealed that the evolution of the gene family is diverged into ten clusters of a constructed phylogenetic tree, of which the cluster is the most prevalent with a higher number of genes. Despite their distinct evolutionary clusters, a significant number of members contains common conserved motifs. The gene family was functionally differentiated into three primary functional categories, biological process, molecular function, and cellular component. Their expression is variable within a tissue, at a developmental stage, and in cultivars. Regardless of the diversity of the functions of genes and evolution, their expression correlated and formed a co-expression gene network. Weighted gene co-expression network analyses identified hub genes that could be regulating ginsenoside biosynthesis. Interestingly, the family also is involved in MeJA regulation. These findings provide a valuable reference for future investigations on genes.