Sánchez-Álvarez Araceli, Quintanilla-Villanueva Gabriela Elizabeth, Rodríguez-Quiroz Osvaldo, Rodríguez-Delgado Melissa Marlene, Villarreal-Chiu Juan Francisco, Sicardi-Segade Analía, Luna-Moreno Donato
Electromecánica Industrial, Universidad Tecnológica de León, Blvd. Universidad Tecnológica #225, Col. San Carlos, León 37670, Guanajuato, Mexico.
Division de Fotónica, Centro de Investigaciones en Óptica AC, Loma del Bosque 115, Col. Lomas del Campestre, León 37150, Guanajuato, Mexico.
Sensors (Basel). 2024 Dec 15;24(24):8008. doi: 10.3390/s24248008.
Methylene blue is a cationic organic dye commonly found in wastewater, groundwater, and surface water due to industrial discharge into the environment. This emerging pollutant is notably persistent and can pose risks to both human health and the environment. In this study, we developed a Surface Plasmon Resonance Biosensor employing a BK7 prism coated with 3 nm chromium and 50 nm of gold in the Kretschmann configuration, specifically for the detection of methylene blue. For the first time, laccases immobilized on a gold surface were utilized as bio-receptors for this organic dye. The enzyme was immobilized using carbodiimide bonds with EDC/NHS crosslinkers, allowing for the analysis of samples with minimal preparation. The method demonstrated validation with a limit of detection (LOD) of 4.61 mg L and a limit of quantification (LOQ) of 15.37 mg L, a working range of 0-100 mg L, and an R value of 0.9614 during real-time analysis. A rainwater sample spiked with methylene blue yielded a recovery rate of 122.46 ± 4.41%. The biosensor maintained a stable signal over 17 cycles and remained effective for 30 days at room temperature.
亚甲蓝是一种阳离子有机染料，由于工业排放到环境中，常见于废水、地下水和地表水中。这种新兴污染物具有显著的持久性，会对人类健康和环境构成风险。在本研究中，我们开发了一种表面等离子体共振生物传感器，采用Kretschmann结构，在BK7棱镜上涂覆3 nm铬和50 nm金，专门用于检测亚甲蓝。首次将固定在金表面的漆酶用作这种有机染料的生物受体。使用碳二亚胺键与EDC/NHS交联剂固定酶，使样品分析的制备过程最少。该方法经验证，检测限（LOD）为4.61 mg/L，定量限（LOQ）为15.37 mg/L，工作范围为0 - 100 mg/L，实时分析时R值为0.9614。添加亚甲蓝的雨水样品回收率为122.46 ± 4.41%。该生物传感器在17个循环中保持稳定信号，在室温下30天内仍有效。
