Kovacs Emerich, Arzang Behnam, Salimi Elham, Butler Michael, Bridges Greg E, Thomson Douglas J
Department of Electrical and Computer Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada.
Department of Microbiology, University of Manitoba, Winnipeg, MB R3T 5V6, Canada.
Sensors (Basel). 2024 Dec 18;24(24):8071. doi: 10.3390/s24248071.
This paper presents a lens-free imaging approach utilizing an array of light sources, capable of measuring the dielectric properties of many particles simultaneously. This method employs coplanar electrodes to induce velocity changes in flowing particles through dielectrophoretic forces, allowing the inference of individual particle properties from differential velocity changes. Both positive and negative forces are detectable. The light source utilized in this system is composed of LEDs with a wavelength of 470 nm, while detection is performed using a 256-element optical array detector. Measurements with 10 μm polystyrene beads demonstrate this method can resolve changes equivalent to a Clausius-Mossotti factor of 0.18. Simulations in this work, using values from the literature, predict that Clausius-Mossotti factor differences of 0.18 are sufficient to differentiate viable from nonviable cells and cancerous from multidrug-resistant cancerous cells. We demonstrate that for Chinese hamster ovary (CHO) cells, the method can collect a dielectric response spectrum for a large number of cells in several minutes. We demonstrate that for CHO cells, Clausius-Mossotti factor differences of 0.18 can be discriminated. Due to its simple detection apparatus and the utilization of high-throughput, wide, clog-resistant channels, this method holds promise for a wide range of applications.
本文提出了一种利用光源阵列的无透镜成像方法，该方法能够同时测量多个颗粒的介电特性。此方法采用共面电极，通过介电泳力在流动颗粒中诱导速度变化，从而能够根据速度差异推断单个颗粒的特性。正负两种力均可检测。该系统中使用的光源由波长为470 nm的发光二极管组成，而检测则使用256元素光学阵列探测器进行。对10μm聚苯乙烯珠的测量表明，该方法能够分辨相当于0.18的克劳修斯 - 莫索蒂因子的变化。本研究中的模拟使用文献中的值预测，0.18的克劳修斯 - 莫索蒂因子差异足以区分活细胞与非活细胞以及癌细胞与多药耐药癌细胞。我们证明，对于中国仓鼠卵巢（CHO）细胞，该方法能够在几分钟内为大量细胞收集介电响应谱。我们证明，对于CHO细胞，0.18的克劳修斯 - 莫索蒂因子差异是可以区分的。由于其简单的检测设备以及对高通量、宽且抗堵塞通道的利用，该方法在广泛的应用中具有前景。
