Achs Adam, Glasa Miroslav, Šubr Zdeno
Department of Virus Ecology, Institute of Virology, Biomedical Research Center of the Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia.
Faculty of Natural Sciences, University of Ss. Cyril and Methodius in Trnava, Námestie J. Herdu 2, 917 01 Trnava, Slovakia.
Viruses. 2024 Dec 15;16(12):1920. doi: 10.3390/v16121920.
Over the past two decades, plant viral vectors have emerged as a powerful tool for the production of recombinant proteins in plants. Among the different plant viruses engineered to carry foreign genes of interest in their genomes, potyviruses have gained attention due to their polyprotein expression strategy and broad host range. To date, at least eleven different species belonging to the genus have been used for heterologous gene expression in both their natural and experimental hosts. This review article provides an overview of the current state of potyvirus-based plant viral vectors, discussing the advantages and limitations of these systems. We also discuss the future challenges and potential applications of potyvirus-based expression vectors, including the production of vaccines, nanoparticles, therapeutics, and metabolic engineering. Overall, we highlight the potential of potyvirus-based vectors as a versatile tool for recombinant protein production in plants.
在过去二十年中，植物病毒载体已成为在植物中生产重组蛋白的强大工具。在经过基因工程改造以在其基因组中携带感兴趣的外源基因的不同植物病毒中，马铃薯Y病毒因其多聚蛋白表达策略和广泛的宿主范围而受到关注。迄今为止，该属中至少有11个不同的物种已被用于在其自然宿主和实验宿主中进行异源基因表达。这篇综述文章概述了基于马铃薯Y病毒的植物病毒载体的现状，讨论了这些系统的优点和局限性。我们还讨论了基于马铃薯Y病毒的表达载体未来面临的挑战和潜在应用，包括疫苗、纳米颗粒、治疗药物的生产以及代谢工程。总体而言，我们强调了基于马铃薯Y病毒的载体作为植物中重组蛋白生产的通用工具的潜力。
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