Brito Gabriela Pereira, Mendonça Graziela Hermínia Andrade, Oliveira Marcos Vinícius Silva De, Reis Aline Chaves, Konig Isaac Filipe Moreira, Thomasi Sérgio Scherrer, Remedio Rafael Neodini
Department of Medicine, Federal University of Lavras, Lavras, Brazil.
Department of Veterinary Medicine, Federal University of Lavras, Lavras, Brazil.
Biotech Histochem. 2025 Jan;100(1):23-31. doi: 10.1080/10520295.2024.2446776. Epub 2025 Jan 8.
Acetylcarvacrol is a semi-synthetic product derived from carvacrol and has known activity against ticks. In vertebrates, the thyroid has been used as a bioindicator in toxicity studies due to its sensitivity to external factors. Thus, the objective of this study was to evaluate the toxic effects of acetylcarvacrol in Wistar rats subjected to repeated dose dermal and oral toxicity tests by means of histopathological analysis of the thyroid. For each test, the rats were divided into 4 groups containing 5 animals. In the topical treatment test, acetylcarvacrol was applied to the trichotomized back of each animal at concentrations of 26, 52 and 104 μL/mL for 21 days. In the oral test, the animals were fed acetylcarvacrol by gavage at concentrations of 26, 52 and 104 μL/mL for 30 days. The control groups were treated only with the vehicles. A significant increase in interstitial tissue vascularization was observed in the group treated topically with the highest concentration of acetylcarvacrol compared to the control. No significant changes were observed between the treatment and control groups in the oral experiment. The comparison between the treated groups and their respective controls also showed no differences in the colloid, the follicle and the follicular cells. The reduced occurrence of changes in this tissue suggests relative safety for use in the control of ticks, although caution is needed when using it at high concentrations or for long periods of time.
乙酰香芹酚是一种从香芹酚衍生而来的半合成产品，已知其具有抗蜱活性。在脊椎动物中，甲状腺因其对外部因素敏感，已被用作毒性研究中的生物指标。因此，本研究的目的是通过对甲状腺的组织病理学分析，评估乙酰香芹酚对接受重复剂量皮肤和口服毒性试验的Wistar大鼠的毒性作用。对于每项试验，将大鼠分为4组，每组5只动物。在局部治疗试验中，将乙酰香芹酚以26、52和104μL/mL的浓度应用于每只动物经脱毛处理的背部，持续21天。在口服试验中，动物通过灌胃给予浓度为26、52和104μL/mL的乙酰香芹酚，持续30天。对照组仅用赋形剂处理。与对照组相比，局部用最高浓度乙酰香芹酚处理的组间组织血管化显著增加。在口服实验中，治疗组和对照组之间未观察到显著变化。治疗组与其各自对照组之间的比较也显示，在胶体、滤泡和滤泡细胞方面没有差异。该组织变化发生率降低表明其在蜱虫控制中的使用相对安全，尽管在高浓度或长时间使用时需要谨慎。
