Yang Qiao, Feng Yaya, Liu Lizhen, Yang Linlin, Wang Mingshu, Wu Ying, Tian Bin, Ou Xumin, Jia Renyong, Zhu Dekang, Chen Shun, Liu Mafeng, Zhao Xinxin, Zhang Shaqiu, Huang Juan, Sun Di, He Yu, Wu Zhen, Zhang Ling, Yu Yanling, Cheng Anchun
Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education, Chengdu, 611130, China.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu City, 611130, Sichuan, China.
Vet Res. 2025 Jan 7;56(1):3. doi: 10.1186/s13567-024-01420-9.
In herpesvirus, the terminase subunit pUL15 is involved in cleavage of the viral genome concatemers in the nucleus. Previous studies have shown that herpes simplex virus 1 (HSV-1) pUL15 can enter the nucleus without other viral proteins and help other terminase subunits enter the nucleus. However, this study revealed that duck plague virus (DPV) pUL15 cannot localize independently to the nucleus and can only be localized in the nucleus in the presence of pUL28 and pUL33. However, the data suggested the presence of a potential nuclear localization signal (NLS) in DPV pUL15, which is important for the localization of the terminase subunits. Subsequently, several single-point mutants were constructed to identify the vital amino acids within the NLS. The conserved amino acids K187, R188, and K190 are critical for the nuclear localization of pUL15, pUL28, and pUL33 but not for their interaction. Furthermore, corresponding recombinant viruses were constructed. The results revealed that the mutations rUL15K187Q, rUL15K188Q and rUL15K190Q had an obvious influence on concatemeric genome cleavage, but only K190Q significantly affected the production of progeny virions. These findings indicate that the NLS is important for the functions of DPV pUL15. Overall, a potential NLS and the key amino acids in DPV pUL15 were identified. Mutations in K187, K188 and K190 affected the cleavage of the concatemeric genome, but only mutations in K190 had an obvious effect on viral proliferation.
在疱疹病毒中，末端酶亚基pUL15参与细胞核内病毒基因组多联体的切割。先前的研究表明，单纯疱疹病毒1型（HSV-1）pUL15可以在没有其他病毒蛋白的情况下进入细胞核，并帮助其他末端酶亚基进入细胞核。然而，本研究表明，鸭瘟病毒（DPV）pUL15不能独立定位于细胞核，只有在pUL28和pUL33存在的情况下才能定位于细胞核。然而，数据表明DPV pUL15中存在潜在的核定位信号（NLS），这对末端酶亚基的定位很重要。随后，构建了几个单点突变体以鉴定NLS内的关键氨基酸。保守氨基酸K187、R188和K190对pUL15、pUL28和pUL33的核定位至关重要，但对它们的相互作用不重要。此外，构建了相应的重组病毒。结果表明，突变体rUL15K187Q、rUL15K188Q和rUL15K190Q对多联体基因组切割有明显影响，但只有K190Q显著影响子代病毒粒子的产生。这些发现表明NLS对DPV pUL15的功能很重要。总体而言，鉴定出了DPV pUL15中的潜在NLS和关键氨基酸。K187、K188和K190的突变影响了多联体基因组的切割，但只有K190的突变对病毒增殖有明显影响。
Zotero 插件
