Sabbatini Paola, Cipriani Sabrina, Biagini Andrea, Sallicandro Luana, Arcuri Cataldo, Romani Rita, Prontera Paolo, Mirarchi Alessandra, Gentile Rosaria, Bianco Diletta Del, Gliozheni Elko, Gerli Sandro, Giardina Irene, Arduini Maurizio, Favilli Alessandro, Malvasi Antonio, Tinelli Andrea, Fioretti Bernard
Department of Chemistry, Biology and Biotechnologies, University of Perugia, Via dell'Elce di Sotto 8, 06123 Perugia, Italy.
Rheumatology Unit, Department of Medicine, School of Medicine, University of Perugia, 06123 Perugia, Italy.
Cells. 2025 Jan 4;14(1):50. doi: 10.3390/cells14010050.
Amniotic fluid is a complex and dynamic biological matrix that surrounds the fetus during the pregnancy. From this fluid, is possible to isolate various cell types with particular interest directed towards stem cells (AF-SCs). These cells are highly appealing due to their numerous potential applications in the field of regenerative medicine for tissues and organs as well as for treating conditions such as traumatic or ischemic injuries to the nervous system, myocardial infarction, or cancer. AF-SCs, when subcultured in the presence of basic Fibroblast Growth Factor (bFGF), have been shown to survive and migrate when transplanted into the striatum of the rat brain, exhibiting behavior characteristics of neuronal/glial progenitor cells. In this work, we performed an electrophysiological characterization to ascertain the propensity of AF-SCs to differentiate into glial and neuronal cells by bFGF. By using patch clamp technique we characterized a fibroblast-like morphology that display a barium-sensitive inward-rectifying potassium current (Kir) and calcium-activated potassium currents (KCa). The electrophysiological and calcium dynamics of histamine, a marker of undifferentiated neural progenitors, was further studied. Histamine promoted intracellular calcium increase by Fura-2 recording and calcium-activated potassium current activation with a similar temporal profile in AF-SC. The data presented in this paper ultimately confirm the expression in AF-SCs of the Kir and KCa currents, also showing regulation by endogenous stimuli such as histamine for the latter.
羊水是一种复杂且动态的生物基质，在孕期环绕着胎儿。从这种液体中，可以分离出各种细胞类型，其中对干细胞（羊水干细胞，AF-SCs）特别感兴趣。这些细胞极具吸引力，因为它们在组织和器官的再生医学领域有众多潜在应用，也可用于治疗诸如神经系统的创伤性或缺血性损伤、心肌梗死或癌症等病症。已表明，当在碱性成纤维细胞生长因子（bFGF）存在的情况下传代培养时，羊水干细胞移植到大鼠脑纹状体后能够存活并迁移，表现出神经元/神经胶质祖细胞的行为特征。在这项工作中，我们进行了电生理特性分析，以确定羊水干细胞在bFGF作用下分化为神经胶质细胞和神经元细胞的倾向。通过使用膜片钳技术，我们对一种成纤维细胞样形态进行了表征，该形态显示出对钡敏感的内向整流钾电流（Kir）和钙激活钾电流（KCa）。我们进一步研究了组胺（一种未分化神经祖细胞的标志物）的电生理和钙动力学。通过Fura-2记录，组胺促进细胞内钙增加，并在羊水干细胞中以类似的时间进程激活钙激活钾电流。本文呈现的数据最终证实了羊水干细胞中Kir和KCa电流的表达，同时也表明了后者受组胺等内源性刺激的调节。
