Wojnicz Dorota, Korzekwa Kamila, Guźniczak Mateusz, Wernecki Maciej, Ulatowska-Jarża Agnieszka, Buzalewicz Igor, Tichaczek-Goska Dorota
Department of Biology and Medical Parasitology, Faculty of Medicine, Wrocław Medical University, Mikulicza-Radeckiego 9, 50-345 Wroclaw, Poland.
Department of Microbiology, Faculty of Biological Sciences, University of Wrocław, Przybyszewskiego 63, 51-148 Wroclaw, Poland.
Int J Mol Sci. 2024 Dec 25;26(1):76. doi: 10.3390/ijms26010076.
Multidrug-resistant bacteria represent a significant challenge in the treatment of bacterial infections, often leading to therapeutic failures. This issue underlines the need to develop strategies that improve the efficacy of conventional antibiotic therapies. In this study, we aimed to assess whether a plant-derived compound, α-mangostin, and photodynamic therapy (PDT) could enhance the antibacterial activity of ciprofloxacin against uropathogenic strains of and . Using nanopore sequencing technology, we confirmed that the clinical strains tested were classified as multidrug-resistant. Digital holotomography (DHT) was used to examine α-mangostin-induced changes in the bacterial cells' penetration by a photosensitizer. A scanning confocal fluorescence microscope was used to visualize photosensitizer penetration into bacterial cells and validate DHT results. A synergistic effect between α-mangostin and ciprofloxacin was observed exclusively in strains, while no enhancement of ciprofloxacin's antibacterial activity was detected in strains when combined with α-mangostin. Notably, photodynamic therapy significantly potentiated the antibacterial effects of ciprofloxacin and its combination with α-mangostin compared to untreated controls. In addition, morphological changes were observed in bacterial cells exposed to these antimicrobials. In conclusion, our findings suggest that α-mangostin and PDT may serve as valuable adjuncts to ciprofloxacin, improving the eradication of uropathogens.
多重耐药菌在细菌感染治疗中构成重大挑战，常导致治疗失败。这一问题凸显了制定提高传统抗生素疗法疗效策略的必要性。在本研究中，我们旨在评估一种植物来源的化合物α - 山竹黄酮以及光动力疗法（PDT）是否能增强环丙沙星对尿路致病性菌株和的抗菌活性。利用纳米孔测序技术，我们确认所测试的临床菌株被归类为多重耐药菌。数字全息断层扫描（DHT）用于检测α - 山竹黄酮诱导的细菌细胞对光敏剂摄取的变化。使用扫描共聚焦荧光显微镜观察光敏剂进入细菌细胞的情况并验证DHT结果。仅在菌株中观察到α - 山竹黄酮与环丙沙星之间的协同效应，而在菌株中，当与α - 山竹黄酮联合使用时，未检测到环丙沙星抗菌活性的增强。值得注意的是，与未处理的对照相比，光动力疗法显著增强了环丙沙星及其与α - 山竹黄酮联合使用的抗菌效果。此外，在暴露于这些抗菌剂的细菌细胞中观察到了形态变化。总之，我们的研究结果表明，α - 山竹黄酮和光动力疗法可能是环丙沙星的有价值辅助手段，有助于提高尿路病原体的根除率。
