Tao Ran, Zhang Jihong, Meng Lixia, Gao Jin, Miao Chaohua, Liu Weixiao, Jin Wujun, Wan Yusong
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Inspection and Testing Center for Ecological and Environmental Risk Assessment of Plant and Plant-Related Microorganisms (Beijing), Ministry of Agriculture and Rural Affairs, Beijing 100081, China.
Int J Mol Sci. 2024 Dec 26;26(1):108. doi: 10.3390/ijms26010108.
Genetically modified (GM) herbicide-tolerant soybean 'Zhonghuang 6106', which introduces a glyphosate-resistant gene, ensures soybean yield while allowing farmers to reduce the use of other herbicides, thereby reducing weed management costs. To protect consumer rights and facilitate government supervision, we have established a simple and rapid on-site nucleic acid detection method for GM soybean 'Zhonghuang 6106'. This method leverages the isothermal amplification characteristics of RPA technology and the high specificity of CRISPR-Cas12a to achieve high sensitivity and accuracy in detecting GM soybean components. By optimizing experimental conditions, the platform can quickly produce visual detection results, significantly reducing detection time and improving efficiency. The system can detect down to 10 copies/μL of 'Zhonghuang 6106' DNA templates, and the entire detection process takes about 1 h. The technology also has strong editing capabilities; by redesigning the primers and crRNA in the method, it can become a specific detection method for other GM samples, providing strong technical support for the regulation and safety evaluation of GM crops.
转基因抗除草剂大豆“中黄6106”导入了抗草甘膦基因，在保证大豆产量的同时，可使农民减少其他除草剂的使用，从而降低杂草管理成本。为保护消费者权益并便于政府监管，我们建立了一种针对转基因大豆“中黄6106”的简便快速的现场核酸检测方法。该方法利用RPA技术的等温扩增特性和CRISPR-Cas12a的高特异性，在检测转基因大豆成分时实现了高灵敏度和准确性。通过优化实验条件，该平台能够快速产生可视化检测结果，显著缩短检测时间并提高效率。该系统能够检测低至10拷贝/μL的“中黄6106”DNA模板，整个检测过程约需1小时。该技术还具有强大的编辑能力；通过重新设计方法中的引物和crRNA，它可以成为其他转基因样品的特异性检测方法，为转基因作物的监管和安全性评价提供有力的技术支持。
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