He Jia-Qi, Yu Dou-Cheng, Ren Si-Yu, Zhang Xiao-Li, Li Xin-Yi, Huang Mei-Juan, Huang Hai-Quan
Research and Development Center of Landscape Plants and Horticulture Flowers, Yunnan Engineering Research Center for Functional Flower Resources and Industrialization, Southwest Research Center for Engineering Technology of Landscape Architecture (State Forestry and Grassland Administration), College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China.
Int J Mol Sci. 2024 Dec 28;26(1):174. doi: 10.3390/ijms26010174.
One of the most important characteristics of ornamental plants is leaf color, which enhances the color of plant landscapes and attracts pollinators for reproduction. The leaves of 'Sakimp005' are initially green, then the middle part appears yellow, then gradually become white, while the edge remains green. In the study, leaves of 'Sakimp005', in four developmental stages (S1-G, S2-C, S3-C, and S4-C), were selected for the determination of pigment content, chromaticity values, integrative metabolomics, and transcriptomics analyses. The carotenoid content of leaves varied significantly and regularly at four stages, and the colorimetric values corroborated the phenotypic observations. The results of integrative metabolomics and transcriptomics analysis show that the accumulation of two carotenoids (lutein and zeaxanthin), to different degrees in the leaves of 'Sakimp005' at four stages, led to the vary yellowing phenomenon. We speculated that the carotenoid biosynthesis (containing two branches: α-branch and β-branch) in leaves by and in the α-branch and genes in the β-branch differed. These findings provide a molecular basis for plants' leaf color breeding and improve the knowledge of the leaf color mechanism.
观赏植物最重要的特征之一是叶片颜色，它能增强植物景观的色彩并吸引传粉者进行繁殖。‘Sakimp005’的叶片最初是绿色，然后中间部分出现黄色，接着逐渐变为白色，而边缘仍保持绿色。在本研究中，选取了‘Sakimp005’处于四个发育阶段（S1-G、S2-C、S3-C和S4-C）的叶片进行色素含量测定、色度值测定、综合代谢组学和转录组学分析。叶片中的类胡萝卜素含量在四个阶段有显著且规律的变化，色度值证实了表型观察结果。综合代谢组学和转录组学分析结果表明，‘Sakimp005’叶片在四个阶段中两种类胡萝卜素（叶黄素和玉米黄质）的不同程度积累导致了叶片变黄现象的差异。我们推测叶片中类胡萝卜素生物合成（包含两个分支：α-分支和β-分支）中α-分支的 和 以及β-分支的 基因存在差异。这些发现为 植物的叶片颜色育种提供了分子基础，并增进了对叶片颜色机制的认识。
