Khan Zesmin, Chowdhury Devasish, Upadhyaya Hrishikesh
Department of Botany, Cotton University, Guwahati, 781001, Assam, India.
Physical Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, 781035, India.
Plant Physiol Biochem. 2025 Mar;220:109470. doi: 10.1016/j.plaphy.2024.109470. Epub 2025 Jan 4.
Selenium nanoparticles are well known for their antioxidant and stress-mitigating properties. In our study, composite nanoformulations of selenium and chitosan have been synthesized. The synthesized composite nanoformulations were 50 nm in diameter, spherical in shape, and had higher antioxidant activities and stability than the selenium and chitosan nanoparticles. In our study, Luit rice seedlings grown in an arsenic-treated Hoagland solution showed a reduction of growth, decreased superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, ascorbate, and glutathione content. Otherwise, superoxide anion, hydrogen peroxide, and malondialdehyde content increased in arsenic-stressed conditions. The alone application of Selenium nanoparticles, chitosan nanoparticles, and their nanoformulation improved growth, reduced stress parameters, and enhanced enzymatic and non-enzymatic activity. Additionally, the reduction of superoxide anion, hydrogen peroxide, and malondialdehyde content was higher by applying composite nanoformulations in arsenic-stressed conditions than selenium and chitosan nanoparticles. The treatment of composite nanoformulation also regulated the enzymatic and non-enzymatic antioxidant activity higher than that of other nanoparticles. It might be due to the higher stability and antioxidant activity of composite nanoformulations than that of selenium and chitosan nanoparticles. Our study suggests that the composite nanoformulation enhanced the growth of rice plants by mitigating arsenic-induced reactive oxygen species and upregulating antioxidant activity.
硒纳米颗粒因其抗氧化和减轻应激的特性而广为人知。在我们的研究中，已合成了硒和壳聚糖的复合纳米制剂。合成的复合纳米制剂直径为50纳米，呈球形，并且具有比硒和壳聚糖纳米颗粒更高的抗氧化活性和稳定性。在我们的研究中，在经砷处理的霍格兰溶液中生长的鲁伊特水稻幼苗表现出生长减缓、超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、愈创木酚过氧化物酶、抗坏血酸和谷胱甘肽含量降低。否则，在砷胁迫条件下，超氧阴离子、过氧化氢和丙二醛含量会增加。单独施用硒纳米颗粒、壳聚糖纳米颗粒及其纳米制剂可促进生长、降低应激参数并增强酶促和非酶促活性。此外，在砷胁迫条件下施用复合纳米制剂比施用硒和壳聚糖纳米颗粒能更有效地降低超氧阴离子、过氧化氢和丙二醛的含量。复合纳米制剂处理还比其他纳米颗粒更能调节酶促和非酶促抗氧化活性。这可能是由于复合纳米制剂比硒和壳聚糖纳米颗粒具有更高的稳定性和抗氧化活性。我们的研究表明，复合纳米制剂通过减轻砷诱导的活性氧并上调抗氧化活性来促进水稻植株的生长。
