Ghoneem Khalid M, Rashad Ehsan M, Al-Askar Abdulaziz A, Helmy Yosra A, El-Gamal Seham M A, Ibrahim Shafik D, Saber WesamEldin I A
Department of Seed Pathology Research, Plant Pathology Research Institute, Agricultural Research Center, (ARC), Giza, 12619, Egypt.
Department of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Heliyon. 2024 Dec 10;11(1):e41081. doi: 10.1016/j.heliyon.2024.e41081. eCollection 2025 Jan 15.
L. faced a new and previously undocumented leaf blight disease for the first time. This disease manifests initially as small, circular, or irregular brown spots on older leaves, which gradually expand and merge into dark brownish blotches over time. This disease's impact is detrimental to plant health and seed quality. Through comprehensive morphological characteristics, and molecular identification (GenBank OL823169), the pathogen was conclusively identified as . Further confirmation was obtained by analyzing target sequences for the gene (GenBank OR492259). Koch's postulates were strictly adhered to, leading to the successful re-isolation of from artificially infected 8-week-old coriander plants (local variety; Balady), providing unequivocal evidence that the fungus is responsible for leaf blight disease, marking the first such confirmation worldwide. The significant activity of fungal enzymes may be associated with pathogenicity. The seed-health test supported the hypothesis that seeds play a central role in the transmission of , as it was detected in 40 % of seed lots, alongside other common pathogenic and saprophytic genera. This study underscores the urgency of implementing seed treatments to curtail the pathogen's spread. The emergence of coriander leaf blight disease documented here (Egypt; 30° 57' 25″ N and 31° 35 ' 54″E) for the first time, necessitates heightened awareness and proactive measures to protect coriander plants all over the world.
L.首次面临一种新的、此前未记录的叶枯病。这种病害最初表现为老叶上的小的圆形或不规则褐色斑点，随着时间的推移，这些斑点会逐渐扩大并融合成深褐色斑块。这种病害对植物健康和种子质量有害。通过综合形态特征和分子鉴定（GenBank登录号OL823169），最终确定病原体为 。通过分析 基因的目标序列（GenBank登录号OR492259）获得了进一步的确认。严格遵循了科赫法则，从人工感染的8周龄香菜植株（当地品种；Balady）中成功再次分离出 ，明确证明这种真菌是叶枯病的病原体，这在全球尚属首次确认。真菌酶的显著活性可能与致病性有关联。种子健康测试支持了种子在 传播中起核心作用这一假说，因为在40%的种子批次中检测到了 ，同时还检测到了其他常见的致病和腐生属。本研究强调了实施种子处理以减少病原体传播的紧迫性。这里（埃及；北纬30°57′25″，东经31°35′54″）首次记录到香菜叶枯病的出现，有必要提高全球对保护香菜植株的认识并采取积极措施。
