Asghar Sumeera, Hayat Faisal, Zhao Zimo, Zheng Zhu, Ghori Nida, Lu Zhang, Li Yan, Chen Chunli
The Key Laboratory of Plant Resources Conservation Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences Institute of Agro-Bioengineering, Guizhou University, Guiyang, 5505, China.
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Planta. 2025 Jan 14;261(2):33. doi: 10.1007/s00425-025-04616-1.
De novo root regeneration (DNRR) involves activation of special cells after wounding, along with the converter cells, reactive oxygen species, ethylene, and jasmonic acid, also playing key roles. An updated DNRR model is presented here with gene regulatory networks. Root formation after tissue injury is a type of plant regeneration known as de novo root regeneration (DNRR). DNRR system has wide applications in agriculture and tissue culture biotechnology. This review summarizes the recent advancements in the DNRR model for the cellular and molecular framework, targeting leaf explant of Arabidopsis and highlighting differences among direct and indirect pathways. Key findings highlight the presence of special cells in leaf explants after wounding, under different time lapses, through single-cell sequencing of the transcriptional landscape. The possible roles of reactive oxygen species (ROS), ethylene, and jasmonic acid are explored in the early establishment of wounding signals (short/long) for auxin biosynthesis, ultimately leading to adventitious root formation. The synergistic manner of 3rd type of special cells along converter and regeneration-competent cells automatically leads towards cell fate transition for auxin flux in regeneration-competent cells. The signaling mechanisms of these suggested special cells need to be further investigated to understand the DNRR mechanistic story entirely, in addition to root-to-root regeneration and stem-to-root regeneration. Meta-analysis of DNRR is also presented for past and future reference.
从头根再生（DNRR）涉及受伤后特殊细胞的激活，同时转化细胞、活性氧、乙烯和茉莉酸也发挥着关键作用。本文提出了一个更新的带有基因调控网络的DNRR模型。组织损伤后的根形成是一种被称为从头根再生（DNRR）的植物再生类型。DNRR系统在农业和组织培养生物技术中有着广泛的应用。本综述总结了DNRR模型在细胞和分子框架方面的最新进展，以拟南芥叶外植体为研究对象，并突出了直接和间接途径之间的差异。主要发现强调了通过对转录图谱进行单细胞测序，在不同时间间隔下受伤叶外植体中存在特殊细胞。探讨了活性氧（ROS）、乙烯和茉莉酸在生长素生物合成的伤口信号（短/长）早期建立中的可能作用，最终导致不定根的形成。第三种特殊细胞与转化细胞和具有再生能力的细胞协同作用，自动导致具有再生能力的细胞中生长素通量的细胞命运转变。除了根对根再生和茎对根再生外，这些建议的特殊细胞的信号机制需要进一步研究，以全面了解DNRR的机制。还对DNRR进行了荟萃分析，以供过去和未来参考。
