He Fayin, Zeng Yue, An Yin, Pan Yangnian, Zhu Jiana, Fei Chunpeng, Tian Guishuang, Yi Yin, Tang Ming, Li Kun, Yang Zhengting
Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
Int J Biol Macromol. 2025 Mar;297:139776. doi: 10.1016/j.ijbiomac.2025.139776. Epub 2025 Jan 12.
ER stress activates the unfolded protein response (UPR), a critical mechanism for maintaining cellular homeostasis in plants. The p24 protein family is known to be involved in protein trafficking between the endoplasmic reticulum (ER) and the Golgi apparatus, but its role in ER stress remains unclear in plants. In this study, we found that Atp24δ8(delta8), a member of the δ-2 subclass of the p24 family, is significantly upregulated in response to tunicamycin-induced ER stress. Subcellular localization confirmed that Atp24δ8 resides in the ER, and CRISPR-Cas9 loss-of-function mutants displayed increased sensitivity to ER stress. Further analysis showed that Atp24δ8 regulates key UPR genes, highlighting its role in promoting ER stress tolerance. These findings provide new insights into the molecular mechanisms that plants use to cope with ER stress, potentially contributing to the development of stress-tolerant crops.
内质网应激激活未折叠蛋白反应（UPR），这是植物维持细胞内稳态的关键机制。已知p24蛋白家族参与内质网（ER）和高尔基体之间的蛋白质转运，但其在植物内质网应激中的作用仍不清楚。在本研究中，我们发现p24家族δ-2亚类成员Atp24δ8在衣霉素诱导的内质网应激反应中显著上调。亚细胞定位证实Atp24δ8定位于内质网，CRISPR-Cas9功能缺失突变体对内质网应激表现出更高的敏感性。进一步分析表明，Atp24δ8调节关键的UPR基因，突出了其在促进内质网应激耐受性方面的作用。这些发现为植物应对内质网应激的分子机制提供了新的见解，可能有助于培育抗逆作物。
