Wang Xiaoying, Cao Lizhi, Chang Rui, Shen Junwei, Ma Linlin, Li Yanfei
Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China.
School of Life Sciences and Technology, Tongji University, Shanghai, China.
iScience. 2024 Dec 13;28(1):111596. doi: 10.1016/j.isci.2024.111596. eCollection 2025 Jan 17.
The intricate development and functionality of the mammalian heart are influenced by the heterogeneous nature of cardiomyocytes (CMs). In this study, single-cell and spatial transcriptomics were utilized to analyze cells from neonatal mouse hearts, resulting in a comprehensive atlas delineating the transcriptional profiles of distinct CM subsets. A continuum of maturation states was elucidated, emphasizing a progressive developmental trajectory rather than discrete stages. This approach enabled the mapping of these states across various cardiac regions, illuminating the spatial organization of CM development and the influence of the cellular microenvironment. Notably, a subset of transitional CMs was identified, characterized by a transcriptional signature marking a pivotal maturation phase, presenting a promising target for therapeutic strategies aimed at enhancing cardiac regeneration. This atlas not only elucidates fundamental aspects of cardiac development but also serves as a valuable resource for advancing research into cardiac physiology and pathology, with significant implications for regenerative medicine.
哺乳动物心脏复杂的发育和功能受到心肌细胞（CMs）异质性的影响。在本研究中，利用单细胞和空间转录组学分析新生小鼠心脏中的细胞，生成了一个全面的图谱，描绘了不同CM亚群的转录谱。阐明了一个连续的成熟状态，强调了渐进的发育轨迹而非离散的阶段。这种方法能够在不同心脏区域绘制这些状态，揭示CM发育的空间组织和细胞微环境的影响。值得注意的是，鉴定出了一个过渡性CM亚群，其特征是具有标志关键成熟阶段的转录特征，为旨在增强心脏再生的治疗策略提供了一个有前景的靶点。这个图谱不仅阐明了心脏发育的基本方面，还为推进心脏生理学和病理学研究提供了宝贵资源，对再生医学具有重要意义。
