一种针对低实验毒力小肠结肠炎耶尔森氏菌菌株组织侵袭性的简易成年小鼠测试。

A simple adult-mouse test for tissue invasiveness in Yersinia enterocolitica strains of low experimental virulence.

作者信息

Bakour R, Balligand G, Laroche Y, Cornelis G, Wauters G

出版信息

J Med Microbiol. 1985 Apr;19(2):237-46. doi: 10.1099/00222615-19-2-237.

Abstract

The virulence of Yersinia enterocolitica depends on the presence of a 70-kilobase plasmid, called the Vwa plasmid. This situation is particularly favourable for studies of the mechanism of pathogenicity, but these are hindered by the lack of a suitable animal test to monitor the virulence of the human-pathogenic strains isolated outside the USA which belong to serogroups O:3, O:9 and O:5,27. We observed that, after oral administration to the mouse, the Vwa-positive strains of these serogroups produce a discrete systemic infection while the Vwa-negative strains do not. We present here a simple mouse-virulence test based on this observation.

摘要

小肠结肠炎耶尔森菌的毒力取决于一种名为Vwa质粒的70千碱基质粒的存在。这种情况对于致病性机制的研究特别有利，但由于缺乏合适的动物试验来监测在美国境外分离出的属于O:3、O:9和O:5,27血清群的人致病菌株的毒力，这些研究受到了阻碍。我们观察到，经口给予小鼠后，这些血清群的Vwa阳性菌株会产生离散的全身感染，而Vwa阴性菌株则不会。基于这一观察结果，我们在此提出一种简单的小鼠毒力试验。