Zhang Wanyi, Peng Qiuqi, Yuan Linhan, Wu Caiwen, Wang Mengqi, Li Hongbo, Li Hongjuan, Yu Jinghua
College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin, China.
J Food Sci. 2025 Jan;90(1):e70010. doi: 10.1111/1750-3841.70010.
Infant formulas are constantly being updated and upgraded, and N-glycans are functional glycans that have not been fully exploited to date. Commercial whey protein materials are often used as basic ingredients in infant formulas. Therefore, it is important to study N-glycans in commercial whey protein materials. We used matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and ion chromatography to analyze N-glycans in bovine lactoferrin (Lf), whey protein isolate (WPI), whey protein concentrate 70 (WPC 70), goat whey protein powder 50, demineralized whey powder 90 (D90), and desalted goat whey powder. The results showed that 30, 6, 28, 16, 8, and 9 N-glycans were found in Lf, D90, desalted goat whey powder, WPI, WPC 70, and goat whey protein powder 50, respectively. A total of four structures of N-glycans were detected in this study. Only bovine Lf and WPC 70 contained fucosylated and sialylated binding (SFN-type) glycan structures. Regarding content, WPC 70 showed the highest yield of 14.5 mg/g, and the degree of sialylation was higher than fucosylation. This study provides a potential basis for the future use of commercial whey protein materials in dairy products such as infant formula.
婴儿配方奶粉不断更新升级，而N-聚糖是迄今尚未得到充分开发的功能性聚糖。商业乳清蛋白原料常被用作婴儿配方奶粉的基本成分。因此，研究商业乳清蛋白原料中的N-聚糖很重要。我们使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）和离子色谱法分析了牛乳铁蛋白（Lf）、乳清蛋白分离物（WPI）、70%乳清蛋白浓缩物（WPC 70）、50%山羊乳清蛋白粉、90%脱盐乳清粉（D90）和脱盐山羊乳清粉中的N-聚糖。结果表明，在Lf、D90、脱盐山羊乳清粉、WPI、WPC 70和50%山羊乳清蛋白粉中分别发现了30、6、28、16、8和9种N-聚糖。本研究共检测到四种N-聚糖结构。只有牛Lf和WPC 70含有岩藻糖基化和唾液酸化结合（SFN型）聚糖结构。在含量方面，WPC 70的产量最高，为14.5毫克/克，唾液酸化程度高于岩藻糖基化程度。本研究为商业乳清蛋白原料未来在婴儿配方奶粉等乳制品中的应用提供了潜在依据。
