Tang Zhouzhou, Li Zhihao, Wu Guofeng, Li Jianjun, Tan Jianye, Zhu Lixin
Department of Spinal Surgery, Zhujiang Hospital, Southern Medical University, 253 Industrial Avenue Central Guangzhou, Guangdong510280, Guangzhou, Guangdong510280, China.
Department of Spinal Surgery, Jingzhou Hospital Affiliated to Yangtze University, Jingzhou, Hubei434020, China.
Sci Rep. 2025 Jan 23;15(1):2891. doi: 10.1038/s41598-025-87246-7.
Osteosarcoma (OS) is the most common primary malignant bone tumor. Recent evidence suggests that the novel long noncoding RNA DHRS4 antisense RNA 1 (DHRS4-AS1) serves an important role in cancer progression and metastasis. However, its function and molecular mechanism in OS remain largely unknown. In the present study, DHRS4-AS1 expression was detected in OS cells by quantitative PCR. Gain- and loss-of-function experiments were conducted to study the effects of DHRS4-AS1 on the proliferation and apoptosis of OS cells. The potential mechanism of DHRS4-AS1 was examined through bioinformatics analysis and rescue experiments. DHRS4-AS1 was downregulated in OS cell lines. DHRS4-AS1 depletion promoted proliferation and inhibited apoptosis in OS cells, whereas DHRS4-AS1 overexpression had the opposite effects. Further research suggested that DHRS4-AS1 inhibited OS progression by regulating the microRNA-362-5p/aminopeptidase puromycin sensitive axis. The present findings suggested that DHRS4-AS1 may serve as a potential therapeutic target for OS.
骨肉瘤（OS）是最常见的原发性恶性骨肿瘤。最近的证据表明，新型长链非编码RNA DHRS4反义RNA 1（DHRS4-AS1）在癌症进展和转移中起重要作用。然而，其在骨肉瘤中的功能和分子机制仍 largely未知。在本研究中，通过定量PCR检测骨肉瘤细胞中DHRS4-AS1的表达。进行功能获得和功能丧失实验以研究DHRS4-AS1对骨肉瘤细胞增殖和凋亡的影响。通过生物信息学分析和挽救实验研究DHRS4-AS1的潜在机制。DHRS4-AS1在骨肉瘤细胞系中表达下调。DHRS4-AS1缺失促进骨肉瘤细胞增殖并抑制其凋亡，而DHRS4-AS1过表达则产生相反的效果。进一步研究表明，DHRS4-AS1通过调节微小RNA-362-5p/嘌呤霉素敏感氨基肽酶轴抑制骨肉瘤进展。本研究结果表明，DHRS4-AS1可能作为骨肉瘤的潜在治疗靶点。
