Wang Xiaorui, Zhu Bo, Li Hanping, Han Jingwan, Wang Xiaolin, Jia Lei, Zhang Bohan, Li Jingyun, Wang Linding, Liu Yongjian, Wen Hongling, Li Lin
Department of Health Inspection and Quarantine, School of Public Health, Cheeloo College of Medicine, Shandong Provincial Key Laboratory for Prevention and Control of Emerging Infectious Diseases and Biosafety in Higher Education Institutions, Shandong Provincial Key Laboratory of Intelligent Monitoring, Early Warning, Prevention and Control for Infectious Diseases, Shandong University, Jinan, Shandong Province, 250012, China.
State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, 100071, China.
BMC Infect Dis. 2025 Jan 22;25(1):104. doi: 10.1186/s12879-025-10461-0.
CRF01_AE and CRF07_BC are the two most prevalent HIV-1 genotypes in China, and the co-circulation of these two genotypes has led to the continuous generation of CRF_0107 viruses in recent years. However, little is known about the origin and spread of CRF_0107 viruses thus far. This study focused on HIV-1 CRF80_0107, which we previously identified among the MSM population in Beijing and Hebei Province, to explore the demographic distribution, transmission links, and temporal-spatial evolutionary features of the HIV-1 CRF80_0107 strain in China.
With the partial pol region fragment of the HIV-1 CRF80_0107 subtype standard sequence as a reference, BLAST was used to search for highly similar sequences in the Los Alamos HIV Sequence Database, followed by preliminary subtype identification via COMET. Further phylogenetic and recombination breakpoint analyses were conducted to verify the subtypes and recombination patterns. We also performed a distance-based molecular network analysis to identify potential relationships among different HIV-positive individuals. In addition, spatiotemporal evolutionary dynamics analysis of the candidate CRF80_0107 sequences was performed via a Bayesian approach.
A total of 36 partial pol gene sequences of HIV-1 CRF80_0107 were identified from 2009 to 2018 from 5 provinces in China. Phylogenetic and spatial-temporal dynamics analyses indicated that CRF80_0107 likely originated in Beijing around 2009 and spread to Guangdong Province around 2012. Population dynamics analysis revealed that CRF80_0107 experienced a significant increase in population size from 2009 to 2011 and then stabilized. The study also found that the number of cases in Guangdong Province was second only to that in Beijing and formed 2 relatively independent transmission clusters in the MSM population in Shenzhen, Guangdong Province.
The HIV-1 CRF80_0107 strain has spread to cities beyond its origin, particularly the MSM population in Shenzhen city, Guangdong Province, which is an area with a high incidence of HIV. This highlights the importance of continuous monitoring for the emergence and dynamic changes of novel HIV-1 recombinant viruses and the necessity of implementing effective preventive measures targeting specific populations in particular regions.
CRF01_AE和CRF07_BC是中国两种最常见的HIV-1基因型，这两种基因型的共同流行导致近年来CRF_0107病毒不断产生。然而，迄今为止，关于CRF_0107病毒的起源和传播知之甚少。本研究聚焦于我们之前在北京和河北省男男性行为人群中鉴定出的HIV-1 CRF80_0107，以探索HIV-1 CRF80_0107毒株在中国的人群分布、传播链以及时空进化特征。
以HIV-1 CRF80_0107亚型标准序列的部分pol区片段为参考，利用BLAST在洛斯阿拉莫斯HIV序列数据库中搜索高度相似序列，随后通过COMET进行初步亚型鉴定。进行进一步的系统发育和重组断点分析以验证亚型和重组模式。我们还进行了基于距离的分子网络分析，以确定不同HIV阳性个体之间的潜在关系。此外，通过贝叶斯方法对候选CRF80_0107序列进行时空进化动力学分析。
2009年至2018年期间，从中国5个省份共鉴定出36条HIV-1 CRF80_0107的部分pol基因序列。系统发育和时空动力学分析表明，CRF80_0107可能于2009年左右起源于北京，并于2012年左右传播至广东省。种群动力学分析显示，CRF80_0107的种群规模在2009年至2011年期间显著增加，然后趋于稳定。该研究还发现，广东省的病例数仅次于北京，并在广东省深圳市的男男性行为人群中形成了2个相对独立的传播簇。
HIV-1 CRF80_0107毒株已传播至其起源地以外的城市，特别是广东省深圳市的男男性行为人群，该地区是HIV高发地区。这凸显了持续监测新型HIV-1重组病毒的出现和动态变化的重要性，以及针对特定地区特定人群实施有效预防措施的必要性。
