Li Min, Wang Wenting, Chen Xiaodan, Lu Xiumei, Huang Yahui
Department of Tea Science, College of Horticulture Science, South China Agricultural University, Guangzhou, 510642, China.
Plant Cell Rep. 2025 Jan 23;44(2):34. doi: 10.1007/s00299-024-03384-8.
Integration of resistance indicators, metabolomes, and transcriptomes to elucidate that there is a positive correlation between disease susceptibility and cold tolerance in tea plants. The flavonoid pathway was found to be the major metabolic and transcriptional enrichment pathway. A key domain NB-ARC was identified through joint analysis, along with analysis of key domains within the NB-ARC protein. Tea is a healthy beverage and the tea plant is a woody plant rich in secondary metabolites. In the face of abnormal climate change year by year, it is important to investigate the mechanisms by which tea plants resist both biotic and abiotic stresses. In this study, we found different tea plant cultivars were evaluated for cold and disease resistance have highly correlated. Subsequently, two cold and fungal resistant cultivars were screened from a Shuixian population that had been cold domesticated for 50 years, and transcriptome and metabolome assays were performed on the two materials under cold and anthracnose stresses, using Baiye Dancong as a control. The analyses found that differential metabolites were most enriched in the flavonoid pathway and differentially expressed genes were most enriched in the pathway related to disease course after pathogen stress and cold stress. Combined metabolome and transcriptome analyses identified 30 genes that were positively correlated with flavonoid content after pathogen stress and cold stress, of which the number of genes with NB-ARC structural domains was 11, which accounted for the largest proportion. These 11 genes with NB-ARC structural domains were analyzed by family analysis and found to be highly involved in different tissues transcriptomes of tea plants, indicating the importance of the NB-ARC structural domains in biotic and abiotic stresses, and providing a theoretical basis of analysis for the subsequent related studies. In this study, through the identification of resistance in different varieties of tea plant and the multi-omics approach, we found the genes related to the key structural domain NB-ARC, which lays the foundation for the study of biologically and abiologically important mechanisms in response to the disease in tea plant.
整合抗性指标、代谢组和转录组，以阐明茶树的疾病易感性与耐寒性之间存在正相关。发现类黄酮途径是主要的代谢和转录富集途径。通过联合分析以及对NB-ARC蛋白内的关键结构域进行分析，鉴定出一个关键结构域NB-ARC。茶是一种健康饮品，茶树是一种富含次生代谢产物的木本植物。面对逐年异常的气候变化，研究茶树抵抗生物和非生物胁迫的机制很重要。在本研究中，我们发现对不同茶树品种的抗寒和抗病性评估具有高度相关性。随后，从经过50年抗寒驯化的水仙群体中筛选出两个抗寒和抗真菌的品种，并以白叶单枞为对照，对这两种材料在低温和炭疽病胁迫下进行转录组和代谢组分析。分析发现，差异代谢物在类黄酮途径中富集最多，差异表达基因在病原体胁迫和低温胁迫后的病程相关途径中富集最多。代谢组和转录组联合分析确定了30个在病原体胁迫和低温胁迫后与类黄酮含量呈正相关的基因，其中具有NB-ARC结构域的基因数量为11个，占比最大。对这11个具有NB-ARC结构域的基因进行家族分析，发现它们高度参与茶树不同组织的转录组，表明NB-ARC结构域在生物和非生物胁迫中的重要性，并为后续相关研究提供了分析的理论基础。在本研究中，通过鉴定不同茶树品种的抗性并采用多组学方法，我们发现了与关键结构域NB-ARC相关的基因，这为研究茶树应对病害的生物和非生物重要机制奠定了基础。
