Ballotari Marco, Truver Michael T, Sojin Nayana A, Agliano Lauren A, Parimoo Rhea, Hoyer Jennifer L, Goodin Amie J, Varma Deepthi S, Chronister Chris W, Roussos-Ross Kay, Goldberger Bruce A
Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville, FL, USA.
Department of Obstetrics & Gynecology, University of Florida College of Medicine, Gainesville, FL, USA.
Anal Bioanal Chem. 2025 Mar;417(8):1507-1518. doi: 10.1007/s00216-025-05738-x. Epub 2025 Jan 23.
An increasing number of cannabis-related products have become available and entered the market, particularly those containing cannabidiol (CBD) and Δ-tetrahydrocannabinol (Δ-THC). Analytical methods for cannabinoids in urine have been described extensively in the literature. However, methods providing good resolution for distinguishing interferences from THC positional isomers are needed. The aim of this project was to develop and validate a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method to quantitate a broad panel of cannabinoids in authentic urine specimens. The method was optimized to quantitate Δ-THC and Δ-THC, 11-OH-Δ-THC and 11-OH-Δ-THC, Δ-THC-COOH and Δ-THC-COOH, CBD, 7-COOH-CBD, CBG, and CBN, and validated with the guidance of the American Academy of Forensic Sciences Standards Board (ASB) Standard 036. The validated assay was then used to evaluate urine samples collected over various time points from female patients (N = 69) enrolled in a study assessing prevalence of marijuana/CBD use during pregnancy from November 2022 to May 2024. Δ- and Δ- isomers were chromatographically resolved and successfully separated. For all analytes, the lower limit of quantitation (LLOQ) was determined to be 10 ng/mL, and the upper limit of quantitation (ULOQ) was 1000 ng/mL. In the authentic samples, the most frequently detected analyte was Δ-THC-COOH, with a median concentration of 278 ng/mL (n = 38). Δ-THC and 11-OH-Δ-THC were detected with a median concentration of 42.4 ng/mL (n = 5) and 65.7 ng/mL (n = 34), respectively. Δ-THC-COOH was detected in n = 3 specimens, with a median concentration of 25.5 ng/mL. The study provided a rapid assay for the analysis of cannabinoids in urine.
越来越多与大麻相关的产品进入市场，尤其是那些含有大麻二酚（CBD）和Δ-四氢大麻酚（Δ-THC）的产品。尿液中大麻素的分析方法在文献中已有广泛描述。然而，需要能够很好地区分THC位置异构体干扰的方法。本项目的目的是开发并验证一种液相色谱-串联质谱（LC-MS/MS）方法，用于定量真实尿液样本中的多种大麻素。该方法经过优化，可定量Δ-THC和Δ-THC、11-OH-Δ-THC和11-OH-Δ-THC、Δ-THC-COOH和Δ-THC-COOH、CBD、7-COOH-CBD、CBG和CBN，并在美国法医科学学会标准委员会（ASB）标准036的指导下进行了验证。然后，使用经过验证的检测方法对2022年11月至2024年5月参与一项评估孕期大麻/ CBD使用情况的研究的女性患者（N = 69）在不同时间点收集的尿液样本进行评估。Δ-和Δ-异构体在色谱上得到了分离并成功分开。对于所有分析物，定量下限（LLOQ）确定为10 ng/mL，定量上限（ULOQ）为1000 ng/mL。在真实样本中，最常检测到的分析物是Δ-THC-COOH，中位浓度为278 ng/mL（n = 38）。检测到的Δ-THC和11-OH-Δ-THC的中位浓度分别为42.4 ng/mL（n = 5）和65.7 ng/mL（n = 34）。在n = 3个样本中检测到了Δ-THC-COOH，中位浓度为25.5 ng/mL。该研究提供了一种快速检测尿液中大麻素的方法。
