Osaili Tareq M, Dhanasekaran Dinesh Kumar, Hasan Fayeza, Obaid Reyad S, Al-Nabulsi Anas A, Olaimat Amin N, Ismail Leila Cheikh, Alkalbani Nadia, Ayyash Mutamed, Bamigbade Gafar Babatunde, Holley Richard, Cheema Adan Shahzadi, Bani Odeh Wael Ahmad, Mohd Khalid Abdulla, Kamal Ayesha Khalid Haji
Department of Clinical Nutrition and Dietetics, College of Health Sciences, University of Sharjah, Sharjah P.O. Box 27272, United Arab Emirates.
Research Institute of Medical & Health Sciences, University of Sharjah, Sharjah P.O. Box 27272, United Arab Emirates.
Foods. 2025 Jan 19;14(2):320. doi: 10.3390/foods14020320.
High-pressure processing (HPP) is used as a non-thermal approach for controlling microbial viability. The purposes of this study were to (i) establish the decimal reduction times (D-values) for pathogenic bacteria during 350 MPa HPP treatment,; (ii) evaluate the impact of 350 MPa HPP on total plate count (TPC), yeasts and molds (YM), and lactic acid bacteria (LAB) in camel milk; (iii) investigate the behavior of several spoilage-causing bacteria during storage at 4 °C and 10 °C for up to 10 d post-HPP treatment; and (iv) assess the effect of HPP on the protein degradation of camel milk. The D-values for , O157:H7, and spp. were 3.77 ± 0.36 min, 1.48 ± 0.08 min, and 2.10 ± 0.13 min, respectively. The HPP treatment decreased pathogenic microorganisms by up to 2 to 3 log cfu/mL (depending on treatment conditions). However, HPP reduced TPC, YM, and LAB by <1 log cfu/mL, regardless of the length of pressure exposure. HPP treatment, even at extended holding times, did not significantly alter either the proteolytic activity or casein micelle structure in camel milk. This study highlights HPP as a promising non-thermal technique for enhancing the microbiological safety of camel milk.
高压处理(HPP)被用作一种控制微生物活力的非热方法。本研究的目的是:(i)确定350兆帕高压处理过程中致病细菌的十进制减少时间(D值);(ii)评估350兆帕高压处理对骆驼奶中总平板计数(TPC)、酵母和霉菌(YM)以及乳酸菌(LAB)的影响;(iii)研究几种致腐细菌在高压处理后于4℃和10℃储存长达10天期间的行为;(iv)评估高压处理对骆驼奶蛋白质降解的影响。大肠杆菌O157:H7和单核细胞增生李斯特菌的D值分别为3.77±0.36分钟、1.48±0.08分钟和2.10±0.13分钟。高压处理使致病微生物减少了2至3个对数cfu/mL(取决于处理条件)。然而,无论压力暴露时间长短,高压处理使总平板计数、酵母和霉菌以及乳酸菌减少了不到1个对数cfu/mL。即使延长保持时间,高压处理也不会显著改变骆驼奶中的蛋白水解活性或酪蛋白胶束结构。本研究强调了高压处理作为一种有前景的非热技术,可提高骆驼奶的微生物安全性。