Gong Zheng, Zhao Hongli, Mao Yi, Zhou Fangfang, Shi Zehui, Lan Minbo
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
Bioelectrochemistry. 2025 Jun;163:108906. doi: 10.1016/j.bioelechem.2025.108906. Epub 2025 Jan 22.
Adenosine plays a crucial role in the cardiovascular and nervous systems of living organisms. Excessive adenosine can lead to arrhythmias or heart failure, making the accurate detection of adenosine highly valuable. Given the widespread use of sensors for detecting small molecules, we propose a sensitive electrochemical aptasensor for adenosine detection in this study. The aptasensor utilized reduced graphene oxide (rGO) as the base material, and its performance was further enhanced by loading gold-platinum nanoparticles (AuPt@rGO) to improve the current response. The AuPt@rGO composite was synthesized using a simple process and was modified onto screen-printed electrodes (SPE) fabricated in the laboratory. This modification increased the effective area of the working electrode, providing more contact sites and enhancing sensitivity for adenosine detection. As a result, the sensor exhibited excellent performance in selectivity, sensitivity, and stability, with a linear response range for adenosine from 10 nM to 10 μM and a detection limit of 1.28 nM (n = 3). Furthermore, successful detection of adenosine levels in real samples was achieved using a portable potentiostat, demonstrating excellent recovery rates (95.99 %-103.4 %). These results indicate the aptasensor's significant potential for future applications in field analysis and medical diagnostics.
腺苷在生物的心血管系统和神经系统中发挥着关键作用。过量的腺苷会导致心律失常或心力衰竭，因此准确检测腺苷具有很高的价值。鉴于用于检测小分子的传感器应用广泛，我们在本研究中提出了一种用于检测腺苷的灵敏电化学适配体传感器。该适配体传感器以还原氧化石墨烯（rGO）为基础材料，并通过负载金铂纳米颗粒（AuPt@rGO）进一步提升其性能，以改善电流响应。AuPt@rGO复合材料采用简单工艺合成，并修饰到实验室制作的丝网印刷电极（SPE）上。这种修饰增加了工作电极的有效面积，提供了更多的接触位点，并提高了对腺苷检测的灵敏度。结果，该传感器在选择性、灵敏度和稳定性方面表现出色，腺苷的线性响应范围为10 nM至10 μM，检测限为1.28 nM（n = 3）。此外，使用便携式恒电位仪成功检测了实际样品中的腺苷水平，回收率极佳（95.99 %-103.4 %）。这些结果表明该适配体传感器在未来的现场分析和医学诊断应用中具有巨大潜力。
