Luo Rongzheng, Zhang Rong, Chen Jiaxuan, Peng Shujun, Sabir Irfan Ali, Li Zhaoqing, Wu Liangfang, Hu Guibing, Shah Kamran, Qin Yonghua
Guangzhou, --- Select One ---, China;
Guangzhou, China;
Plant Dis. 2025 Jan 30. doi: 10.1094/PDIS-08-24-1589-RE.
Pitaya canker disease, caused by , is the primary threat to pitaya cultivation, significantly compromising fruit quality and reducing yield. WRKY transcription factors are essential regulators in plant pathogen recognition and defense mechanisms, yet their specific roles in the development of pitaya canker disease remain largely unexplored. In this study, five genes (, , , , and ) associated with pitaya canker disease were identified through RNA-Seq analysis. The expression levels of and were upregulated following infection. Transient transformation revealed that these five genes negatively influenced the resistance of leaves to canker disease, while promoting the accumulation of reactive oxygen species (ROS) and inducing cell death. Yeast one-hybrid and dual-luciferase reporter assays revealed that HmeWRKY33 directly activated the expression of , while HmeWRKY51 directly inhibited the expression of and , co-participating in regulating the susceptibility of 'Youcihuanglong' pitaya to canker. These findings provide a theoretical basis for breeding new canker-resistant pitaya varieties through genetic transformation.
火龙果溃疡病由 引起，是火龙果种植的主要威胁，严重影响果实品质并降低产量。WRKY转录因子是植物病原体识别和防御机制中的关键调节因子，但其在火龙果溃疡病发生发展中的具体作用仍 largely unexplored。本研究通过RNA-Seq分析鉴定了5个与火龙果溃疡病相关的基因（ 、 、 、 和 ）。 在 感染后， 和 的表达水平上调。瞬时转化表明，这5个基因对 叶片对溃疡病的抗性产生负面影响，同时促进活性氧（ROS）积累并诱导细胞死亡。酵母单杂交和双荧光素酶报告基因检测表明，HmeWRKY33直接激活 的表达，而HmeWRKY51直接抑制 和 的表达，共同参与调节‘优赐黄龙’火龙果对溃疡病的易感性。这些发现为通过基因转化培育新的抗溃疡火龙果品种提供了理论依据。
