INTRODUCTION

The tools to distinguish relapse from reinfection are needed in malaria-endemic areas. We evaluated seroprevalence against sets of specific peptides to the block 2 region of -merozoite surface protein-1 (PvMSP1) to detect parasite clones.

METHODS

We applied amplicon deep sequencing (ADS) of block 2 region of the MSP-1 gene () to determine cocirculating parasite clones within eight -infected individuals. Based on this, a seroprevalence of IgM and IgG antibodies against sets of peptides of different block-2 haplotypes was validated. After, we evaluated the seroprevalence in plasma of 72 pregnant women, from which 31 had recurrent infections.

RESULTS

ADS revealed one block 2 haplotype clone infecting five of eight -infected individuals. In all, IgM antibodies, not IgG, recognized only a set of peptides specific to the block 2 haplotype determined by ADS. In the other three patients, ADS determined three concurrent block 2 haplotype clones, among whom there was always one haplotype that predominated with more than 95% of high-quality reads and two other smaller haplotypes with up to 5% and the least was <1%. We observed higher IgM levels against haplotype-specific peptides corresponding to the predominant clone. The seroprevalence of pregnant women showed that anti-haplotype-specific IgM detected coinfection with parasite clones per pregnant woman and we also observed levels of anti-haplotype-specific IgM in primary infection increased in some recurrent episodes.

CONCLUSION

IgM against sets of peptides specific to different haplotypes may be employed as a serological marker for parasite clones in vivax malaria.