Recombination and pathogenicity analysis of NADC30-like and QYYZ-like PRRSV strains in South China.

Since 2010, the Lineage 1 (NADC30-like) and Lineage 3 (QYYZ-like) strains of PRRSV-2 have become widespread in China, undergoing recombination and resulting in varying virulent and immune-evading mutants that have severely impacted the pig farming industry. In this study, we isolated one NADC30-like strain (GXHX20211106) and one QYYZ-like strain (GXGG202007) from pig serum samples collected in southern China. Comparative genomic analysis revealed that GXHX20211106 shares 90 % identity with NADC30, while GXGG202007 shows 91.4 % similarity to QYYZ. Both strains exhibit characteristic amino acids deletions or insertions in the Nsp2 region. Phylogenetic analyses further classified GXHX20211106 within Lineage 1.8 (NADC30-like) and GXGG202007 in Lineage 3 (QYYZ-like). Recombination analysis demonstrated that GXHX20211106 resulted from recombination between NADC30-like and HP-PRRSV-like strains, while GXGG202007 originated from recombination events involving QYYZ-like, VR-2332-like, and HP-PRRSV-like strains. Pathogenicity studies in piglets indicated that both isolates caused moderate clinical signs, with GXHX20211106 showing higher virulence compared to GXGG202007. No deaths were recorded in the infected piglets. These findings highlight the critical role of recombination in shaping PRRSV virulence and underscore the need for ongoing surveillance and control measures to mitigate the impact of recombinant PRRSV strains in southern China.