Zeng Qi, Liu Xifan, Yan Xuemei, Zhang Jiahao, Li Chao, Yan Chengtai, Zhang Yanfeng, Kliebenstein Daniel, Li Baohua
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Hybrid Rapeseed Research Center of Shaanxi Province, Yangling, Shaanxi, China.
Mol Plant Pathol. 2025 Feb;26(2):e70058. doi: 10.1111/mpp.70058.
Necrotrophic pathogens cause serious threats to agricultural crops, and understanding the resistance genes and their genetic networks is key to breeding new plant cultivars with better resistance traits. Although Alternaria alternata causes black spot in important leafy brassica vegetables, and leads to significant loss of yield and food quality, little is known about plant-A. alternata interactions. In this study, we used a unique and large collection of single, double and triple mutant lines of defence metabolite regulators in Arabidopsis to explore how these transcription factors and their epistatic networks may influence A. alternata infections. This identified nine novel regulators and 20 pairs of epistatic interactions that modulate Arabidopsis plants' defence responses to A. alternata infection. We further showed that the glucosinolate 4-methoxy-indol-3-ylmethyl is the only glucosinolate consistently responsive to A. alternata infection in Col-0 ecotype. With the further exploration of the regulators and the genetic networks on modulating the accumulation of glucosinolates under A. alternata infection, an inverted triangle regulatory model was proposed for Arabidopsis plants' defence responses at a metabolic level and a phenotypic level.
坏死营养型病原菌对农作物构成严重威胁，了解抗性基因及其遗传网络是培育具有更好抗性性状的新植物品种的关键。虽然链格孢菌会导致重要叶用芸苔属蔬菜发生黑斑病，并导致产量和食品质量大幅下降，但人们对植物与链格孢菌的相互作用知之甚少。在本研究中，我们利用拟南芥中防御代谢物调节因子的独特且大量的单突变、双突变和三突变体系，来探究这些转录因子及其上位网络如何影响链格孢菌的感染。这确定了九个新的调节因子和20对上位相互作用，它们调节拟南芥植物对链格孢菌感染的防御反应。我们进一步表明，4-甲氧基吲哚-3-基甲基芥子油苷是Col-0生态型中唯一对链格孢菌感染持续有反应的芥子油苷。随着对链格孢菌感染下调节芥子油苷积累的调节因子和遗传网络的进一步探索，提出了一个关于拟南芥植物在代谢水平和表型水平上防御反应的倒三角调节模型。
