一种分别负载药物和二氢卟吩e6以实现光动力-化学联合治疗的纳米药物递送系统。
A nano drug delivery system loading drugs and chlorin e6 separately to achieve photodynamic-chemo combination therapy.
作者信息
Yin Guohao, Zhao Hongli, Lan Minbo
机构信息
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.
Research Center of Analysis and Test, East China University of Science and Technology, Shanghai, People's Republic of China.
出版信息
Nanomedicine (Lond). 2025 Mar;20(6):559-570. doi: 10.1080/17435889.2025.2460960. Epub 2025 Feb 4.
AIM
To develop a new drug delivery system (DDS) that can load chemotherapy agents and photosensitizer chlorin e6 (Ce6) onto the pores and surfaces of mesoporous silica nanoparticle (MSN) separately.
METHODS
Doxorubicin (DOX) was loaded into the pores of MSNs. Then, polyethyleneimine (PEI) was used to coat the surface of MSN to protect DOX, and then manganese dioxide (MnO) nanoparticles were loaded through adding potassium permanganate (KMnO) to bind with Ce6. Finally, polydopamine (PDA) was coated and coupled with hyaluronic acid (HA).
RESULTS
The synthesized versatile nanoparticle was pH-sensitive and exhibited positive photodynamic therapy (PDT) performance. Besides, it could be observed that the nanoparticles were efficiently taken up by tumor cells through confocal laser scanning microscopy (CLSM) and flow cytometry. Additionally, in vitro experiments suggested that the nanoparticles had pleasing toxicity to various tumor cells and equally positive therapeutic effect when curcumin replaced DOX.
CONCLUSION
Our work suggests that the nanoparticles designed by our strategy have satisfactory combination therapy performance and can enable more chemotherapy drugs to be used in photodynamic-chemo combination therapy.
目的
开发一种新的药物递送系统（DDS），该系统能够将化疗药物和光敏剂二氢卟吩e6（Ce6）分别负载到介孔二氧化硅纳米颗粒（MSN）的孔隙和表面上。
方法
将阿霉素（DOX）负载到MSN的孔隙中。然后，使用聚乙烯亚胺（PEI）包覆MSN表面以保护DOX，接着通过添加高锰酸钾（KMnO）与Ce6结合来负载二氧化锰（MnO）纳米颗粒。最后，包覆聚多巴胺（PDA）并与透明质酸（HA）偶联。
结果
合成的多功能纳米颗粒对pH敏感，并表现出积极的光动力疗法（PDT）性能。此外，通过共聚焦激光扫描显微镜（CLSM）和流式细胞术可以观察到纳米颗粒被肿瘤细胞有效摄取。另外，体外实验表明，当姜黄素替代DOX时，纳米颗粒对各种肿瘤细胞具有良好的毒性和同样积极的治疗效果。
结论
我们的工作表明，通过我们的策略设计的纳米颗粒具有令人满意的联合治疗性能，并且能够使更多的化疗药物用于光动力 - 化疗联合治疗。
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