痘苗病毒自身代谢途径的终产物对其胸苷激酶的抑制作用。
Inhibition of vaccinia virus thymidine kinase by the distal products of its own metabolic pathway.
作者信息
Hruby D E
出版信息
Virus Res. 1985 Mar;2(2):151-6. doi: 10.1016/0168-1702(85)90245-x.
PMID:3993232
Abstract
Vaccinia virus thymidine kinase activity is inhibited by low concentrations (10 microM) of dTDP or dTTP, but not by dTMP. This inhibition is specified for the thymidine nucleotides as dATP, dGTP, and dCTP have no effect. The viral enzyme phosphorylates thymidine to dTMP with typical first-order kinetics. However, evidence was obtained to indicate that the observed dTTP inhibition was noncompetitive in nature. This suggests that thymidine and dTTP interact with different sites in the native viral enzyme.
摘要
痘苗病毒胸苷激酶活性受到低浓度（10微摩尔）的dTDP或dTTP抑制，但不受dTMP抑制。这种抑制作用是胸苷核苷酸特有的，因为dATP、dGTP和dCTP没有影响。该病毒酶以典型的一级动力学将胸苷磷酸化为dTMP。然而，有证据表明观察到的dTTP抑制本质上是非竞争性的。这表明胸苷和dTTP与天然病毒酶中的不同位点相互作用。
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