Grigoreva Tatyana A, Kindt Daria N, Sagaidak Aleksandra V, Novikova Daria S, Tribulovich Vyacheslav G
Laboratory of Molecular Pharmacology, St. Petersburg State Institute of Technology (Technical University), 190013 St. Petersburg, Russia.
Cells. 2025 Jan 22;14(3):170. doi: 10.3390/cells14030170.
Oncological diseases consistently occupy leading positions among the most life-threatening diseases, including in highly developed countries. At the same time, the second most common cause of cancer death is colorectal cancer. The current level of research shows that the development of effective therapy, in this case, requires a new grade of understanding processes during the emergence and development of a tumor. In particular, the concept of cancer stem cells that ensure the survival of chemoresistant cells capable of giving rise to new tumors is becoming widespread. To provide adequate conditions that reproduce natural processes typical for tumor development, approaches based on increasingly complex cellular systems are being improved. This review discusses the main strategies that allow for the study of the properties of tumor cells with an emphasis on colorectal cancer stem cells. The features of working with tumor cells and the advantages and disadvantages of 2D and 3D culture systems are considered.
肿瘤疾病在包括高度发达国家在内的最危及生命的疾病中一直占据领先地位。与此同时，结直肠癌是癌症死亡的第二大常见原因。当前的研究水平表明，在这种情况下，开发有效的治疗方法需要对肿瘤发生和发展过程有新的认识。特别是，癌症干细胞的概念正在广泛传播，这种细胞能够确保产生新肿瘤的化疗耐药细胞的存活。为了提供能够重现肿瘤发展典型自然过程的适当条件，基于日益复杂的细胞系统的方法正在不断改进。本综述讨论了主要策略，这些策略有助于研究肿瘤细胞的特性，重点是结直肠癌干细胞。文中还考虑了处理肿瘤细胞的特点以及二维和三维培养系统的优缺点。
