外源褪黑素通过调节饲料大豆幼苗的抗氧化防御系统和光合效率提高耐旱性。

Exogenous Melatonin Improves Drought Tolerance by Regulating the Antioxidant Defense System and Photosynthetic Efficiency in Fodder Soybean Seedings.

作者信息

Zhao Qianhan, Zheng Xueling, Wang Chen, Wang Qinyi, Wei Qiyun, Liu Xiashun, Liu Yujiao, Chen Along, Jiang Jia, Zhao Xueying, He Tiantian, Qi Jiayi, Han Yuchen, Qin Haonan, Xie Fuchun, Chen Yajun

机构信息

College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.

College of Horticulture, Northeast Agricultural University, Harbin 150030, China.

出版信息

Plants (Basel). 2025 Feb 5;14(3):460. doi: 10.3390/plants14030460.

DOI:10.3390/plants14030460

PMID:39943023

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11819762/

Abstract

Fodder soybean ( L.) with high protein and yield is a popular forage grass in northeast China. Seasonal drought inhibits its growth and development during seedling stage. The objective of this study was to observe morpho-physiological changes in fodder soybean seedlings under melatonin (MT) treatments and identify appropriate concentration to alleviate the drought damage. Two varieties commonly used in northeast China were treated with 0, 50, 100, and 150 μM melatonin at soil water content of 30%. The results indicated that applying melatonin enhanced height, biomass and altered root morphology of fodder soybean seedlings under water-deficient conditions. The treatments with melatonin at different concentrations significantly reduced the contents of HO, O and MDA, while boosting the capacity of the antioxidant defense system and the content of osmotic adjustment substances. Meanwhile, increases in light energy capture and transmission efficiency were observed. Furthermore, treatment with melatonin regulated the expression levels of genes associated with photosynthesis and the antioxidant defense system. Notably, 100 μM melatonin treatment produced the most favorable effect in all treatments under drought conditions. These research results provide new information for enhancing the drought tolerance of fodder soybean using chemical measures.

摘要

高蛋白、高产的秣食豆是中国东北地区一种受欢迎的饲草。季节性干旱会抑制其苗期的生长发育。本研究的目的是观察褪黑素（MT）处理下秣食豆幼苗的形态生理变化，并确定缓解干旱危害的适宜浓度。在中国东北地区常用的两个品种在土壤含水量为30%的条件下分别用0、50、100和150 μM褪黑素进行处理。结果表明，在水分亏缺条件下，施用褪黑素可提高秣食豆幼苗的株高、生物量并改变根系形态。不同浓度褪黑素处理显著降低了过氧化氢（HO）、超氧阴离子（O）和丙二醛（MDA）的含量，同时增强了抗氧化防御系统的能力和渗透调节物质的含量。与此同时，观察到光能捕获和传递效率有所提高。此外，褪黑素处理调节了与光合作用和抗氧化防御系统相关基因的表达水平。值得注意的是，在干旱条件下，100 μM褪黑素处理在所有处理中产生的效果最为理想。这些研究结果为利用化学措施提高秣食豆的耐旱性提供了新的信息。