Kim Young-Won, Jeong Seohyun, Yang Ju-Hee, Tark Dongseob, Kim Woo Hyun, Yang Hyoung-Seok, Mun Seong-Hwan, Kang Sung Hyun, Ko Eun-A, Ko Jae-Hong
Department of Physiology, College of Medicine, Chung-Ang University, Seoul 06974, Korea.
Laboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Korea.
J Anim Sci Technol. 2025 Jan;67(1):69-85. doi: 10.5187/jast.2025.e10. Epub 2025 Jan 31.
Influenza A virus (FLUAV) causes serious diseases in both poultry and humans. Various host proteins, including Mx1, are considered candidates for avian influenza (AI) resistance. After infecting Jeju Native chicken embryo fibroblasts (CEFs) with three types of AI viruses, we performed gene expression profiling, identified single nucleotide polymorphisms (SNPs) through RNA-sequencing, and confirmed phenotypes showing antiviral activity . Highly pathogenic AI viruses upregulated , and and downregulated , , and , while a low pathogenicity AI upregulated , , and and downregulated , , and . However, no virus affected expression. Although SNPs in could not discriminate antiviral activity alone, the only CEF resistant to H5N6, strain AN4, contained the Mx1 631 R/R genotype and strongly expressed an oligoadenylate synthetase-like (OASL) variant with a unique SNP: c.G880A (p.E294K). Using transfected cell lines, H5N6-infected cells expressing with the c.G880A SNP showed minimal cytopathic effects and the lowest gene expression. This study confirms that Jeju Native chickens with specific SNP combinations in both and showed H5N6 resistance and demonstrates the interplay of genetic factors in host-pathogen dynamics, suggesting a need for integrated analyses of multiple resistance genes to inform AI prevention strategies.
甲型流感病毒（FLUAV）可导致禽类和人类患上严重疾病。包括Mx1在内的多种宿主蛋白被认为是抗禽流感（AI）的候选蛋白。在用三种甲型流感病毒感染济州本地鸡胚成纤维细胞（CEF）后，我们进行了基因表达谱分析，通过RNA测序鉴定了单核苷酸多态性（SNP），并确认了具有抗病毒活性的表型。高致病性甲型流感病毒上调了 ， 以及 ，并下调了 ， 以及 ，而低致病性甲型流感病毒上调了 ， 以及 ，并下调了 ， 以及 。然而，没有病毒影响 表达。尽管 中的SNP单独无法区分抗病毒活性，但唯一对H5N6毒株AN4具有抗性的CEF含有Mx1 631 R/R基因型，并强烈表达一种具有独特SNP：c.G880A（p.E294K）的寡腺苷酸合成酶样（OASL）变体。使用转染细胞系，表达具有c.G880A SNP的 的H5N6感染细胞显示出最小的细胞病变效应和最低的 基因表达。本研究证实，在 和 中具有特定SNP组合的济州本地鸡表现出对H5N6的抗性，并证明了遗传因素在宿主 - 病原体动态中的相互作用，表明需要对多个抗性基因进行综合分析，以为禽流感预防策略提供信息。
