Schator Daniel, Kumar Naren G, Chong Samuel Joseph U, Jung Timothy K, Jedel Eric, Smith Benjamin E, Evans David J, Fleiszig Suzanne M J
Herbert Wertheim School of Optometry & Vision Science, University of California, Berkeley, CA, USA.
Graduate Program in Infectious Diseases & Immunity, University of California, Berkeley, CA, USA.
bioRxiv. 2025 Feb 9:2025.02.09.637186. doi: 10.1101/2025.02.09.637186.
is a Gram-negative opportunistic pathogen able to cause life- and sight-threating infections. Once considered an extracellular pathogen, numerous studies have shown it can survive intracellularly. Previously, we showed that inside cells can diversify into distinct subpopulations in vacuoles and the cytoplasm. Here, we report that the transition from vacuoles to cytoplasm requires collaboration with the extracellular subpopulation, through Ca influx enabled by their type III secretion system (T3SS) translocon pore proteins. Moreover, we show that collaboration among subpopulations can contribute to disseminating intracellular bacteria in a mouse infection model. This study provides the basis for future studies to investigate how cooperation of extracellular and intracellular bacteria within the host may contribute to disease progression and persistence.
是一种革兰氏阴性机会致病菌，能够引起危及生命和视力的感染。曾经被认为是一种胞外病原体，大量研究表明它能够在细胞内生存。此前，我们发现其在细胞内可分化为液泡和细胞质中的不同亚群。在此，我们报告从液泡到细胞质的转变需要与胞外亚群协作，通过其三型分泌系统（T3SS）转运孔蛋白促成的钙离子内流来实现。此外，我们表明亚群间的协作有助于在小鼠感染模型中传播细胞内细菌。本研究为未来研究宿主内胞外和胞内细菌的合作如何促进疾病进展和持续存在提供了基础。
