Baraibar M A, Schoning P
J Forensic Sci. 1985 Apr;30(2):439-47.
Frozen tissues were studied histologically to determine what changes were produced by freezing. Samples of brain, lung, liver, small intestine, and kidney from 20 stray dogs were treated in 3 ways: formalin fixation (control), frozen for 2 days plus formalin fixation, or frozen for 7 days plus formalin fixation. Major histological changes caused by freezing were loss of staining, extracellular fluid accumulation, cell shrinkage, fractures, hemolysis, and hematin formation. Lesser changes included loss of bronchial cilia, prominence of collagen in alveolar septa and meninges, and intracellular vacuolization of epithelial cells. Although these changes were annoying, adequate visualization of the tissues was usually possible.
对冷冻组织进行组织学研究，以确定冷冻会产生哪些变化。从20只流浪狗身上采集脑、肺、肝、小肠和肾的样本，采用三种方式进行处理：福尔马林固定（对照）、冷冻2天加福尔马林固定、或冷冻7天加福尔马林固定。冷冻引起的主要组织学变化包括染色丧失、细胞外液积聚、细胞收缩、断裂、溶血和含铁血黄素形成。较小的变化包括支气管纤毛丧失、肺泡间隔和脑膜中胶原突出以及上皮细胞内空泡化。尽管这些变化令人烦恼，但通常仍能对组织进行充分观察。
