The Transcription Factor Regulates Capsaicinoid Biosynthesis in Pepper Fruits.

Pepper is an important vegetable and economic crop, and the MYB family is one of the most numerous transcription factor families in plants, extensively participating in various biological processes such as plant growth, development, and stress resistance. In this study, is identified as a differentially expressed gene in the pepper fruit, and is expressed with higher expression levels in the placenta and pericarp at different development stages of pepper fruit. Analysis of the promoter -elements revealed that this gene contains not only core elements but also environmental factor response elements and plant hormone response elements. The silencing of could reduce the capsaicinoid accumulation in pepper fruit, while the overexpression of could increase capsaicinoid accumulation. Additionally, silencing or overexpressing could regulate the expression of structural genes involved in capsaicinoid biosynthesis. In addition, through yeast one-hybrid experiments, we identified an interaction between and the capsaicinoid biosynthesis structural gene . Further evidence from EMSA experiments and dual luciferase assays confirmed that can bind to the -element ACCAACAACCAAA in the promoter. These results indicate that may regulate the synthesis of capsaicinoids by modulating structural genes in the capsaicinoid biosynthesis pathway, providing new insights into the regulatory mechanisms of capsaicinoid synthesis.