高压均质对模拟胃肠消化过程中桃汁酚类物质谱、抗氧化活性、α-葡萄糖苷酶抑制活性及胰岛素抵抗的影响

Effects of high-pressure homogenization on phenolics profile, antioxidant activity, α-glucosidase inhibitory activity, and insulin resistance of peach juice during simulated gastrointestinal digestion.

作者信息

Peng Yijin, Bie Shenke, Cai Shengbao, Zhou Linyan, Guo Chaofan

机构信息

Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan Province, 650500, China.

Yunnan Engineering Research Center for Fruit & Vegetable Products, Kunming, Yunnan Province, 650500, China.

出版信息

Food Chem X. 2025 Feb 4;26:102263. doi: 10.1016/j.fochx.2025.102263. eCollection 2025 Feb.

DOI:10.1016/j.fochx.2025.102263

PMID:40007514

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11851229/

Abstract

This study underscores the potential of high-pressure homogenization (HPH) as a non-thermal processing technique to improve the bioavailability and health benefits of phenolic compounds in peach juice. Following HPH treatment at 300 MPa, the retention of total phenolic and flavonoid content after gastrointestinal digestion increased by 28 % and 20 %, respectively. The bioactivities of peach juice were significantly improved, as evidenced by enhanced antioxidant activity, with DPPH and ABTS free radical scavenging capacities rising by 8.58 % - 26.68 %, and α-glucosidase inhibition improving by 21.40 % - 32.98 %. Furthermore, glucose consumption in insulin-resistant HepG2 cells increased by 17.15 % - 30.00 %. Molecular docking analysis further revealed that key phenolic compounds, including phlorizin and quercetin, interacted effectively with IRS1/PI3K/AKT pathway proteins, contributing to the alleviation of insulin resistance. These findings highlighted HPH as an innovative strategy for developing functional fruit-based beverages with enhanced nutritional and therapeutic value.

摘要

本研究强调了高压均质化（HPH）作为一种非热加工技术在提高桃汁中酚类化合物的生物利用度和健康益处方面的潜力。在300兆帕的HPH处理后，胃肠道消化后总酚和黄酮含量的保留率分别提高了28%和20%。桃汁的生物活性显著提高，抗氧化活性增强证明了这一点，DPPH和ABTS自由基清除能力提高了8.58% - 26.68%，α-葡萄糖苷酶抑制率提高了21.40% - 32.98%。此外，胰岛素抵抗的HepG2细胞中的葡萄糖消耗增加了17.15% - 30.00%。分子对接分析进一步表明，包括根皮苷和槲皮素在内的关键酚类化合物与IRS1/PI3K/AKT通路蛋白有效相互作用，有助于缓解胰岛素抵抗。这些发现突出了HPH作为一种创新策略，用于开发具有更高营养和治疗价值的功能性水果基饮料。