Dong Tianyu, Shu Yujie, Wang Ying, Yao Mingdong, Xiao Wenhai
Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
Frontier Research Institute for Synthetic Biology, Tianjin University, Tianjin, 300072, China.
Synth Syst Biotechnol. 2025 Jan 26;10(2):495-503. doi: 10.1016/j.synbio.2025.01.007. eCollection 2025 Jun.
, a safe yeast, efficiently metabolizes lipids for the production of food additives and agricultural products. Boosting its growth and lipid utilization capabilities is crucial to enhancing the overall efficiency . Herein, an integrated strategy was implemented to enhance lipid uptake, accumulation and metabolism and systematically promote the growth and lipid utilization of the commonly used Po1f strain. The engineered strain had a specific growth rate of 0.32 h and a lipid content of 67.66 % (g/g DCW), which were 54 % and 26 % greater than those of the original strain. -Carotene was used to verify the production of lipophilic natural compounds, and the highest yield was obtained 48 h earlier using the engineered strain compared to the original strain when consuming same carbon source. These findings show promise in using engineered for rapid growth and improved lipid utilization to boost efficiency of lipophilic product production.
一种安全的酵母能高效代谢脂质以生产食品添加剂和农产品。提高其生长和脂质利用能力对于提高整体效率至关重要。在此，实施了一种综合策略来增强脂质摄取、积累和代谢，并系统地促进常用的Po1f菌株的生长和脂质利用。工程菌株的比生长速率为0.32 h，脂质含量为67.66%（g/g DCW），分别比原始菌株高54%和26%。使用β-胡萝卜素验证亲脂性天然化合物的生产，在消耗相同碳源时，与原始菌株相比，使用工程菌株可提前48小时获得最高产量。这些发现表明，利用工程酵母实现快速生长和提高脂质利用以提高亲脂性产品生产效率具有前景。
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