供体肾脏低温和常温机器灌注期间微生物污染的风险有限。
Limited Risk of Microbial Contamination During Hypothermic and Normothermic Machine Perfusion of Donor Kidneys.
作者信息
Slagter Julia S, Schurink Carolina A M, Chong Ga-Lai M, Reinders Marlies E J, Porte Robert J, Minnee Robert C
机构信息
Department of Surgery, Erasmus MC Transplant Institute, Division of HPB and Transplant Surgery, Erasmus Medical Center Rotterdam, Rotterdam, the Netherlands.
Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center Rotterdam, Rotterdam, the Netherlands.
出版信息
Transpl Infect Dis. 2025 May-Jun;27(3):e70019. doi: 10.1111/tid.70019. Epub 2025 Mar 6.
BACKGROUND
Both hypothermic machine perfusion (HMP) and normothermic machine perfusion (NMP) are increasingly used for preservation of deceased donor kidneys. However, especially NMP can pose as a risk for microbial contamination of the kidney graft as the 37°C perfusate can act as a breeding ground for microbial contaminants.
METHODS
In this study, we investigated the cultures of HMP and NMP perfusates of deceased donor kidneys.
RESULTS
Between January 2021 and April 2024, a total of 99 perfusates were examined (73 HMP and 26 NMP perfusates)-. We found that contamination of HMP perfusate was common, occurring in 21 of 73 cultures (29%). Most bacteria originated from the skin. Microbial contamination during NMP was rare, occurring only in 2 of 26 cultures (8%). Microbial contamination during machine perfusion did not lead to infectious complications in the recipients.
CONCLUSION
Machine perfusion poses a very limited risk of infection in kidney transplant recipients.
背景
低温机器灌注（HMP）和常温机器灌注（NMP）都越来越多地用于 deceased 供体肾脏的保存。然而，特别是 NMP 可能会对肾移植造成微生物污染风险，因为 37°C 的灌注液可成为微生物污染物的滋生地。
方法
在本研究中，我们调查了 deceased 供体肾脏的 HMP 和 NMP 灌注液的培养情况。
结果
在 2021 年 1 月至 2024 年 4 月期间，共检查了 99 份灌注液（73 份 HMP 灌注液和 26 份 NMP 灌注液）。我们发现 HMP 灌注液污染很常见，73 份培养物中有 21 份出现污染（29%）。大多数细菌源自皮肤。NMP 期间的微生物污染很少见，26 份培养物中仅 2 份出现污染（8%）。机器灌注期间的微生物污染未导致受者出现感染并发症。
结论
机器灌注对肾移植受者造成感染的风险非常有限。
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