Li Xinyuan, Munir Maira, Zeng Weiying, Sun Zudong, Chang Xiaoli, Yang Wenyu
College of Agronomy and Sichuan Engineering Research Center for Crop Strip Intercropping System, Sichuan Agricultural University, Chengdu, Sichuan, China.
Institute of Economic Crops, Guangxi Academy of Agricultural Science, Nanning, Guangxi, China.
Front Plant Sci. 2025 Feb 25;16:1540003. doi: 10.3389/fpls.2025.1540003. eCollection 2025.
The family of membrane-bound fatty acid desaturase () genes play a vital role in plant growth, development, and stress responses. The seed-borne pathogen causes seed decay disease during pre-harvest and post-harvest stages of soybean, leading to a significant reduction in yield and quality. Therefore, it is very meaningful to characterize the diversity and function of the gene family in soybean and to elucidate their roles in seed resistance to
In this study, 30 full-length genes were identified from the soybean genome. A range of analysis was conducted to characterize gene and protein structures, chromosomal locations, conserved motif and conserved structural domains, and results showed that genes were clustered into seven subfamilies (, , , , , , ), which is also supported by phylogenetic analysis. The diversity and expansion of the gene family were mainly caused by segmental duplication, and their encoding proteins were observed to locate in chloroplast or endoplasmic reticulum. The promoters of genes contained a set of cis-acting elements in response to plant hormone, defense and stress, light, and plant growth and development, indicating these genes have the complex expression regulation and diverse functions. Gene ontology (GO) and KEGG enrichment pathway analyses showed that genes were closely related to the biosynthesis and metabolism of lipid and unsaturated fatty acids (UFAs). In addition, the expression of was significantly changed in soybean seeds when challenged by the seed decay pathogen . Specifically, , , and genes displayed distinct temporal expression patterns in the resistant ND25 and susceptible CX12, highlighting their potential roles in soybean resistance against infection.
Our findings contribute to a deeper understanding of the gene family and their intricate roles in soybean resistance against the seed-borne pathogen . Moreover, several distinct genes provide valuable candidates for further application in soybean resistant breeding.
膜结合脂肪酸去饱和酶（）基因家族在植物生长、发育和应激反应中起着至关重要的作用。种子传播病原体在大豆收获前和收获后的阶段引发种子腐烂病，导致产量和品质显著下降。因此，表征大豆中该基因家族的多样性和功能，并阐明它们在种子抗（病原体）方面的作用具有重要意义。
在本研究中，从大豆基因组中鉴定出30个全长基因。进行了一系列分析以表征基因和蛋白质结构、染色体定位、保守基序和保守结构域，结果表明这些基因被聚类为七个亚家族（、、、、、、），系统发育分析也支持这一结果。该基因家族的多样性和扩张主要由片段重复引起，并且观察到它们编码的蛋白质定位于叶绿体或内质网。这些基因的启动子包含一组响应植物激素、防御和应激、光以及植物生长发育的顺式作用元件，表明这些基因具有复杂的表达调控和多样的功能。基因本体（GO）和KEGG富集途径分析表明，这些基因与脂质和不饱和脂肪酸（UFA）的生物合成和代谢密切相关。此外，当受到种子腐烂病原体挑战时，大豆种子中该基因的表达发生了显著变化。具体而言，、和基因在抗性品种ND25和感病品种CX12中表现出不同的时间表达模式，突出了它们在大豆抗感染中的潜在作用。
我们的研究结果有助于更深入地了解该基因家族及其在大豆抗种子传播病原体中的复杂作用。此外，几个不同的基因可为大豆抗性育种的进一步应用提供有价值的候选基因。
