一种根际细菌分泌蛋白通过IAA34-PUCHI途径诱导侧根发育。

A rhizobacterium-secreted protein induces lateral root development through the IAA34-PUCHI pathway.

作者信息

Fu Yansong, Liu Yunpeng, Chen Yu, Xiao Jintao, Xie Yuanming, Miao Youzhi, Xu Zhihui, Zhang Nan, Xun Weibing, Xuan Wei, Shen Qirong, Zhang Ruifu

机构信息

Key Lab of Organic-based Fertilizers of China and Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China.

State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

出版信息

Cell Rep. 2025 Mar 25;44(3):115414. doi: 10.1016/j.celrep.2025.115414. Epub 2025 Mar 11.

DOI:10.1016/j.celrep.2025.115414

PMID:40073017

Abstract

Lateral roots (LRs) can continuously forage water and nutrients from soil. In Arabidopsis thaliana, LR development depends on a canonical auxin signaling pathway involving the core transcription factors INDOLE-3-ACETIC ACIDs (IAAs) and AUXIN RESPONSE FACTORs (ARFs). In this study, we identified a protein, bacillolysin, secreted by the beneficial rhizobacterium Bacillus velezensis SQR9, that is able to stimulate LR formation of Arabidopsis. The receptor protein kinase C-TERMINALLY ENCODED PEPTIDE RECEPTOR2 (CEPR2) interacts with bacillolysin and plays a critical role in LR development. In the bacillolysin-regulated signaling pathway, the transcriptional repressor IAA34 interacts with PUCHI to activate downstream LATERAL ORGAN BOUNDARIES-DOMAIN33 (LBD33) expression, consequently inducing LR development. This study reveals interkingdom communication via a protein that mediates a novel pathway to induce LR development.

摘要

侧根（LRs）能够持续从土壤中获取水分和养分。在拟南芥中，侧根发育依赖于一个经典的生长素信号通路，该通路涉及核心转录因子吲哚-3-乙酸（IAAs）和生长素响应因子（ARFs）。在本研究中，我们鉴定出一种由有益根际细菌贝莱斯芽孢杆菌SQR9分泌的蛋白质——芽孢杆菌溶素，它能够刺激拟南芥侧根的形成。受体蛋白激酶C末端编码肽受体2（CEPR2）与芽孢杆菌溶素相互作用，并在侧根发育中起关键作用。在芽孢杆菌溶素调节的信号通路中，转录抑制因子IAA34与PUCHI相互作用，激活下游的侧器官边界结构域33（LBD33）表达，从而诱导侧根发育。这项研究揭示了通过一种蛋白质介导的跨物种交流，该蛋白质介导了一条诱导侧根发育的新途径。