Shi Miaomiao, Zhang Muhan, Bian Huan, Wang Daoying, Xu Weimin, Wei Suhuan, Guo Ruirui
Jiangsu Key Laboratory for Food Quality and Safety State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China.
Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Foods. 2025 Feb 26;14(5):796. doi: 10.3390/foods14050796.
Medium-chain triglycerides (MCTs) have been known to have multiple health benefits in treating metabolic disorders and reducing the incidence of obesity. In the present study, the partial replacement of lard with MCTs assisted by ultrasound treatment on the emulsifying stability and adsorption behavior of myofibrillar protein (MP) was investigated. The results revealed that ultrasound-assisted MCT emulsion had better emulsifying activity and emulsion stability than other groups. MCTs with ultrasound treatment considerably lowered the particle size, facilitated the formation of much smaller and more homogeneous emulsion droplets, and enhanced the oxidative stability of the emulsion. The emulsion had a pseudo-plastic behavior determined through static and dynamic rheological studies, and the MCT emulsion exhibited a larger viscosity and a greater storage modulus (G') compared with the lard emulsion. MCTs could promote protein adsorption levels at the O/W interface, forming a dense interfacial protein film. The surface hydrophobicity and reactive sulfhydryl content increased, accompanied by the transformation of α-helix and β-turn structure to β-sheet and random coil structure, indicating MCTs combined with ultrasound-induced unfolding and crosslinking of MP at the interface. The results suggested that MCTs may have the potential to enhance emulsifying properties in emulsion-type meat products.
中链甘油三酯(MCTs)在治疗代谢紊乱和降低肥胖发生率方面具有多种健康益处,这已为人所知。在本研究中,研究了在超声处理辅助下用MCTs部分替代猪油对肌原纤维蛋白(MP)乳化稳定性和吸附行为的影响。结果表明,超声辅助的MCT乳液比其他组具有更好的乳化活性和乳液稳定性。经超声处理的MCTs显著降低了粒径,促进形成更小且更均匀的乳液滴,并提高了乳液的氧化稳定性。通过静态和动态流变学研究确定该乳液具有假塑性行为,与猪油乳液相比,MCT乳液表现出更大的粘度和更高的储能模量(G')。MCTs可促进蛋白质在油/水界面的吸附水平,形成致密的界面蛋白膜。表面疏水性和活性巯基含量增加,同时α-螺旋和β-转角结构转变为β-折叠和无规卷曲结构,表明MCTs与超声诱导的MP在界面处展开和交联相结合。结果表明,MCTs可能具有增强乳化型肉制品乳化特性的潜力。