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不同大型藻类上附生细菌群落的全年定量、结构和动态揭示了一个持久的核心微生物群和强烈的宿主特异性。

Year-Round Quantification, Structure and Dynamics of Epibacterial Communities From Diverse Macroalgae Reveal a Persistent Core Microbiota and Strong Host Specificities.

作者信息

Brunet Maéva, Le Duff Nolwen, Barbeyron Tristan, Thomas François

机构信息

Sorbonne Université, CNRS, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff (SBR), Roscoff, France.

出版信息

Environ Microbiol Rep. 2025 Apr;17(2):e70077. doi: 10.1111/1758-2229.70077.

Abstract

Macroalgae-bacteria interactions play pivotal ecological roles in coastal ecosystems. Previous characterisation of surface microbiota from various macroalgae evidenced fluctuations based on host tissues, physicochemical and environmental parameters. However, the dynamics and degree of similarity of epibacterial communities colonising phylogenetically distant algae from the same habitat are still elusive. We conducted a year-long monthly epimicrobiota sampling on five algal species inhabiting an English Channel rocky shore: Laminaria digitata, Ascophyllum nodosum, Fucus serratus (brown algae), Palmaria palmata (red alga) and Ulva sp. (green alga). To go beyond relative compositional data and estimate absolute variations in taxa abundance, we combined qPCR measurements of 16S rRNA gene copies with amplicon metabarcoding. A core microbiome composed of 10 genera was consistently found year-round on all algae. Notably, the abundant genus Granulosicoccus stood out for being the only one present in all samples and displayed an important microdiversity. Algal host emerged as the primary driver of epibacterial community composition, before seasonality, and bacterial taxa specifically associated with one or several algae were identified. Moreover, the impact of seasons on the epimicrobiota varied depending on algal tissues. Overall, this study provides an extensive characterisation of the microbiota of intertidal macroalgae and enhances our understanding of algal-bacteria holobionts.

摘要

大型藻类与细菌的相互作用在沿海生态系统中发挥着关键的生态作用。先前对各种大型藻类表面微生物群的表征表明,其会基于宿主组织、物理化学和环境参数而发生波动。然而,定殖在来自同一栖息地的系统发育关系较远的藻类上的附生细菌群落的动态变化和相似程度仍不清楚。我们对生活在英吉利海峡岩石海岸的五种藻类进行了为期一年的每月一次的附生微生物采样,这五种藻类分别是:掌状海带、泡叶藻、锯齿墨角藻(褐藻)、红掌藻(红藻)和石莼属(绿藻)。为了超越相对组成数据并估计分类群丰度的绝对变化,我们将16S rRNA基因拷贝的qPCR测量与扩增子元条形码分析相结合。在所有藻类上全年都一致发现了一个由10个属组成的核心微生物群。值得注意的是,丰富的颗粒球菌属脱颖而出,它是所有样本中唯一存在的属,并且表现出重要的微多样性。在季节性因素之前,藻类宿主成为附生细菌群落组成的主要驱动因素,并且鉴定出了与一种或几种藻类特异性相关的细菌分类群。此外,季节对附生微生物群的影响因藻类组织而异。总体而言,这项研究对潮间带大型藻类的微生物群进行了广泛的表征,并增进了我们对藻类 - 细菌共生体的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8605/11903338/690febd5a8f0/EMI4-17-e70077-g005.jpg

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